Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 41394..41919 Replicon plasmid unnamed2
Accession NZ_CP022914
Organism Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CJZ79_RS25860 Protein ID WP_001159871.1
Coordinates 41394..41699 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag CJZ79_RS25865 Protein ID WP_000829078.1
Coordinates 41701..41919 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ79_RS25835 36548..37677 + 1130 Protein_39 IS110 family transposase -
CJZ79_RS25840 37930..39160 - 1231 Protein_40 IS66 family transposase -
CJZ79_RS25845 39370..39678 - 309 WP_047646152.1 hypothetical protein -
CJZ79_RS25850 39765..40409 - 645 WP_001144037.1 ParA family protein -
CJZ79_RS25855 40587..41393 - 807 WP_000016960.1 site-specific integrase -
CJZ79_RS25860 41394..41699 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CJZ79_RS25865 41701..41919 - 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CJZ79_RS25880 42441..42698 + 258 WP_000343095.1 hypothetical protein -
CJZ79_RS25885 42698..43240 + 543 WP_032328642.1 hypothetical protein -
CJZ79_RS25890 43484..44326 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
CJZ79_RS25895 44329..45417 + 1089 WP_064734538.1 putative hexosyltransferase CapU -
CJZ79_RS25900 45422..46372 + 951 WP_032328199.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - etpB / etpA / espC / eltB / eltA / cs3 1..117021 117021


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T80154 WP_001159871.1 NZ_CP022914:c41699-41394 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80154 NZ_CP022914:c41699-41394 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT80154 WP_000829078.1 NZ_CP022914:c41919-41701 [Escherichia coli O6:H16]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80154 NZ_CP022914:c41919-41701 [Escherichia coli O6:H16]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References