Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4396578..4397075 Replicon chromosome
Accession NZ_CP022912
Organism Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CJZ79_RS23205 Protein ID WP_001173343.1
Coordinates 4396578..4396751 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag CJZ79_RS23210 Protein ID WP_071597404.1
Coordinates 4396779..4397075 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ79_RS23195 4393243..4394700 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CJZ79_RS23200 4394937..4396454 + 1518 WP_001295074.1 lysine--tRNA ligase -
CJZ79_RS23205 4396578..4396751 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CJZ79_RS23210 4396779..4397075 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CJZ79_RS23220 4397302..4397574 - 273 WP_000405647.1 N-acetyltransferase -
CJZ79_RS23225 4397586..4397816 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CJZ79_RS23230 4397997..4399628 + 1632 WP_001216490.1 sensor histidine kinase -
CJZ79_RS23235 4399625..4400344 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4385364..4445306 59942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T80147 WP_001173343.1 NZ_CP022912:c4396751-4396578 [Escherichia coli O6:H16]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T80147 NZ_CP022912:c4396751-4396578 [Escherichia coli O6:H16]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT80147 WP_071597404.1 NZ_CP022912:c4397075-4396779 [Escherichia coli O6:H16]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT80147 NZ_CP022912:c4397075-4396779 [Escherichia coli O6:H16]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References