Detailed information of TA system TA080133

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1014570..1015224
• Replicon: chromosome
• Accession: NZ_CP022912
• Organism: Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: CJZ79_RS05435
• Protein ID: WP_000244781.1
• Coordinates: 1014817..1015224 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: CJZ79_RS05430
• Protein ID: WP_047646062.1
• Coordinates: 1014570..1014836 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ79_RS05410	1010658..1012091	-	1434	WP_001394742.1	6-phospho-beta-glucosidase BglA	-
CJZ79_RS05415	1012136..1012447	+	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
CJZ79_RS05420	1012611..1013270	+	660	WP_000250274.1	hemolysin III family protein	-
CJZ79_RS05425	1013347..1014327	-	981	WP_000886087.1	tRNA-modifying protein YgfZ	-
CJZ79_RS05430	1014570..1014836	+	267	WP_047646062.1	FAD assembly factor SdhE	Antitoxin
CJZ79_RS05435	1014817..1015224	+	408	WP_000244781.1	protein YgfX	Toxin
CJZ79_RS05440	1015264..1015785	-	522	WP_001055874.1	flavodoxin FldB	-
CJZ79_RS05445	1015897..1016793	+	897	WP_000806650.1	site-specific tyrosine recombinase XerD	-
CJZ79_RS05450	1016818..1017528	+	711	WP_000715224.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CJZ79_RS05455	1017534..1019267	+	1734	WP_032328035.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T80133 WP_000244781.1 NZ_CP022912:1014817-1015224 [Escherichia coli O6:H16]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T80133 NZ_CP022912:1014817-1015224 [Escherichia coli O6:H16]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10503.11 Da
• Isoelectric Point: 5.6375

>AT80133 WP_047646062.1 NZ_CP022912:1014570-1014836 [Escherichia coli O6:H16]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSADEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT80133 NZ_CP022912:1014570-1014836 [Escherichia coli O6:H16]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGCTGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PAM6