Detailed information of TA system TA080101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2222972..2223501
• Replicon: chromosome
• Accession: NZ_CP022908
• Organism: Staphylococcus aureus strain 545

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CGQ00_RS11830
• Protein ID: WP_000621175.1
• Coordinates: 2222972..2223334 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CGQ00_RS11835
• Protein ID: WP_000948331.1
• Coordinates: 2223331..2223501 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGQ00_RS11805	2219949..2220719	-	771	Protein_2177	RNA polymerase sigma factor SigB	-
CGQ00_RS11810	2220694..2221173	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CGQ00_RS11815	2221175..2221501	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CGQ00_RS11820	2221621..2222622	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CGQ00_RS11830	2222972..2223334	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CGQ00_RS11835	2223331..2223501	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CGQ00_RS11840	2223586..2224734	-	1149	WP_001281145.1	alanine racemase	-
CGQ00_RS11845	2224800..2225159	-	360	WP_000581200.1	holo-ACP synthase	-
CGQ00_RS11850	2225163..2225654	-	492	WP_001205910.1	PH domain-containing protein	-
CGQ00_RS11855	2225641..2227224	-	1584	WP_001294631.1	PH domain-containing protein	-
CGQ00_RS11860	2227217..2227696	-	480	WP_001287088.1	hypothetical protein	-
CGQ00_RS11865	2227905..2228465	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T80101 WP_000621175.1 NZ_CP022908:c2223334-2222972 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T80101 NZ_CP022908:c2223334-2222972 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT80101 WP_000948331.1 NZ_CP022908:c2223501-2223331 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT80101 NZ_CP022908:c2223501-2223331 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1