Detailed information of TA system TA080083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180421..2180950
• Replicon: chromosome
• Accession: NZ_CP022906
• Organism: Staphylococcus aureus strain 546

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CGP99_RS11510
• Protein ID: WP_000621175.1
• Coordinates: 2180421..2180783 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CGP99_RS11515
• Protein ID: WP_000948331.1
• Coordinates: 2180780..2180950 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGP99_RS11485	2177398..2178168	-	771	Protein_2112	RNA polymerase sigma factor SigB	-
CGP99_RS11490	2178143..2178622	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CGP99_RS11495	2178624..2178950	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CGP99_RS11500	2179070..2180071	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CGP99_RS11510	2180421..2180783	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CGP99_RS11515	2180780..2180950	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CGP99_RS11520	2181035..2182183	-	1149	WP_001281145.1	alanine racemase	-
CGP99_RS11525	2182249..2182608	-	360	WP_000581200.1	holo-ACP synthase	-
CGP99_RS11530	2182612..2183103	-	492	WP_001205910.1	PH domain-containing protein	-
CGP99_RS11535	2183090..2184673	-	1584	WP_001294631.1	PH domain-containing protein	-
CGP99_RS11540	2184666..2185145	-	480	WP_001287088.1	hypothetical protein	-
CGP99_RS11545	2185354..2185914	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T80083 WP_000621175.1 NZ_CP022906:c2180783-2180421 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T80083 NZ_CP022906:c2180783-2180421 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT80083 WP_000948331.1 NZ_CP022906:c2180950-2180780 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT80083 NZ_CP022906:c2180950-2180780 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1