Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2139244..2139773 Replicon chromosome
Accession NZ_CP022900
Organism Staphylococcus aureus strain 468

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag CGP96_RS10905 Protein ID WP_000621176.1
Coordinates 2139244..2139606 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag CGP96_RS10910 Protein ID WP_000948331.1
Coordinates 2139603..2139773 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGP96_RS10880 2136223..2136993 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
CGP96_RS10885 2136968..2137447 - 480 WP_001190825.1 anti-sigma B factor RsbW -
CGP96_RS10890 2137449..2137775 - 327 WP_001052491.1 anti-sigma factor antagonist -
CGP96_RS10895 2137894..2138895 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
CGP96_RS10905 2139244..2139606 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CGP96_RS10910 2139603..2139773 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CGP96_RS10915 2139858..2141006 - 1149 WP_001281140.1 alanine racemase -
CGP96_RS10920 2141072..2141431 - 360 WP_000581197.1 holo-ACP synthase -
CGP96_RS10925 2141435..2141926 - 492 WP_001286801.1 PH domain-containing protein -
CGP96_RS10930 2141919..2143496 - 1578 WP_001294646.1 PH domain-containing protein -
CGP96_RS10935 2143489..2143968 - 480 WP_001287082.1 hypothetical protein -
CGP96_RS10940 2144177..2144737 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T79981 WP_000621176.1 NZ_CP022900:c2139606-2139244 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T79981 NZ_CP022900:c2139606-2139244 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT79981 WP_000948331.1 NZ_CP022900:c2139773-2139603 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT79981 NZ_CP022900:c2139773-2139603 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References