Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2139189..2139718 Replicon chromosome
Accession NZ_CP022899
Organism Staphylococcus aureus strain 143

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag CGP95_RS10905 Protein ID WP_000621176.1
Coordinates 2139189..2139551 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag CGP95_RS10910 Protein ID WP_000948331.1
Coordinates 2139548..2139718 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGP95_RS10880 2136168..2136938 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
CGP95_RS10885 2136913..2137392 - 480 WP_001190825.1 anti-sigma B factor RsbW -
CGP95_RS10890 2137394..2137720 - 327 WP_001052491.1 anti-sigma factor antagonist -
CGP95_RS10895 2137839..2138840 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
CGP95_RS10905 2139189..2139551 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CGP95_RS10910 2139548..2139718 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CGP95_RS10915 2139803..2140951 - 1149 WP_001281140.1 alanine racemase -
CGP95_RS10920 2141017..2141376 - 360 WP_000581197.1 holo-ACP synthase -
CGP95_RS10925 2141380..2141871 - 492 WP_001286801.1 PH domain-containing protein -
CGP95_RS10930 2141864..2143441 - 1578 WP_001294646.1 PH domain-containing protein -
CGP95_RS10935 2143434..2143913 - 480 WP_001287082.1 hypothetical protein -
CGP95_RS10940 2144122..2144682 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T79965 WP_000621176.1 NZ_CP022899:c2139551-2139189 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T79965 NZ_CP022899:c2139551-2139189 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT79965 WP_000948331.1 NZ_CP022899:c2139718-2139548 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT79965 NZ_CP022899:c2139718-2139548 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References