Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2810171..2810807 Replicon chromosome
Accession NZ_CP022891
Organism Bacillus subtilis strain DKU_NT_03

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CJZ71_RS14860 Protein ID WP_003156187.1
Coordinates 2810457..2810807 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag CJZ71_RS14855 Protein ID WP_003225183.1
Coordinates 2810171..2810452 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ71_RS14835 2806530..2807129 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
CJZ71_RS14840 2807224..2807589 + 366 WP_014478897.1 holo-ACP synthase -
CJZ71_RS14845 2807755..2808771 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
CJZ71_RS14850 2808886..2810055 + 1170 WP_014478898.1 alanine racemase -
CJZ71_RS14855 2810171..2810452 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CJZ71_RS14860 2810457..2810807 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CJZ71_RS14865 2810923..2811747 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
CJZ71_RS14870 2811752..2812117 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
CJZ71_RS14875 2812121..2812522 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
CJZ71_RS14880 2812534..2813541 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
CJZ71_RS14885 2813610..2813939 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
CJZ71_RS14890 2813936..2814418 + 483 WP_003234299.1 anti-sigma B factor RsbW -
CJZ71_RS14895 2814384..2815172 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
CJZ71_RS14900 2815172..2815771 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T79852 WP_003156187.1 NZ_CP022891:2810457-2810807 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T79852 NZ_CP022891:2810457-2810807 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT79852 WP_003225183.1 NZ_CP022891:2810171-2810452 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT79852 NZ_CP022891:2810171-2810452 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References