Detailed information of TA system TA079852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2810171..2810807
• Replicon: chromosome
• Accession: NZ_CP022891
• Organism: Bacillus subtilis strain DKU_NT_03

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CJZ71_RS14860
• Protein ID: WP_003156187.1
• Coordinates: 2810457..2810807 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: CJZ71_RS14855
• Protein ID: WP_003225183.1
• Coordinates: 2810171..2810452 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ71_RS14835	2806530..2807129	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
CJZ71_RS14840	2807224..2807589	+	366	WP_014478897.1	holo-ACP synthase	-
CJZ71_RS14845	2807755..2808771	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
CJZ71_RS14850	2808886..2810055	+	1170	WP_014478898.1	alanine racemase	-
CJZ71_RS14855	2810171..2810452	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CJZ71_RS14860	2810457..2810807	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CJZ71_RS14865	2810923..2811747	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
CJZ71_RS14870	2811752..2812117	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
CJZ71_RS14875	2812121..2812522	+	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
CJZ71_RS14880	2812534..2813541	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
CJZ71_RS14885	2813610..2813939	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
CJZ71_RS14890	2813936..2814418	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
CJZ71_RS14895	2814384..2815172	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
CJZ71_RS14900	2815172..2815771	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T79852 WP_003156187.1 NZ_CP022891:2810457-2810807 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T79852 NZ_CP022891:2810457-2810807 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT79852 WP_003225183.1 NZ_CP022891:2810171-2810452 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT79852 NZ_CP022891:2810171-2810452 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32