Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3334859..3335495 Replicon chromosome
Accession NZ_CP022890
Organism Bacillus subtilis strain DKU_NT_02

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CJZ70_RS17610 Protein ID WP_003156187.1
Coordinates 3334859..3335209 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag CJZ70_RS17615 Protein ID WP_003225183.1
Coordinates 3335214..3335495 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ70_RS17570 3329896..3330495 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
CJZ70_RS17575 3330495..3331283 - 789 WP_095010755.1 RNA polymerase sigma factor SigB -
CJZ70_RS17580 3331249..3331731 - 483 WP_095010756.1 anti-sigma B factor RsbW -
CJZ70_RS17585 3331728..3332057 - 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
CJZ70_RS17590 3332126..3333133 - 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
CJZ70_RS17595 3333145..3333546 - 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
CJZ70_RS17600 3333550..3333915 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
CJZ70_RS17605 3333920..3334744 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
CJZ70_RS17610 3334859..3335209 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CJZ70_RS17615 3335214..3335495 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CJZ70_RS17620 3335611..3336780 - 1170 WP_015252766.1 alanine racemase -
CJZ70_RS17625 3336895..3337911 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
CJZ70_RS17630 3338077..3338442 - 366 WP_015252768.1 holo-ACP synthase -
CJZ70_RS17635 3338537..3339127 + 591 WP_095010757.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T79851 WP_003156187.1 NZ_CP022890:c3335209-3334859 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T79851 NZ_CP022890:c3335209-3334859 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT79851 WP_003225183.1 NZ_CP022890:c3335495-3335214 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT79851 NZ_CP022890:c3335495-3335214 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References