Detailed information of TA system TA079825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 800..1359
• Replicon: chromosome
• Accession: NZ_CP022879
• Organism: Brucella abortus strain MC

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D7H3C9
• Locus tag: CJP70_RS00015
• Protein ID: WP_002964098.1
• Coordinates: 800..1045 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: CJP70_RS00020
• Protein ID: WP_002964097.1
• Coordinates: 1045..1359 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJP70_RS00015	800..1045	+	246	WP_002964098.1	BrnT family toxin	Toxin
CJP70_RS00020	1045..1359	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
CJP70_RS00030	1707..1877	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
CJP70_RS00035	2225..2934	-	710	Protein_4	porin family protein	-
CJP70_RS00040	3280..3756	-	477	WP_002966806.1	hypothetical protein	-
CJP70_RS00045	3779..4054	-	276	WP_002964092.1	hypothetical protein	-
CJP70_RS00050	4051..4281	-	231	WP_002964091.1	hypothetical protein	-
CJP70_RS00055	4278..4996	-	719	Protein_8	hypothetical protein	-
CJP70_RS00060	5046..5249	+	204	WP_002964088.1	hypothetical protein	-
CJP70_RS00065	5246..5461	+	216	WP_002971459.1	hypothetical protein	-
CJP70_RS00070	5464..5667	+	204	WP_002964087.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9196.83 Da
• Isoelectric Point: 10.3158

>T79825 WP_002964098.1 NZ_CP022879:800-1045 [Brucella abortus]
MKIFWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T79825 NZ_CP022879:800-1045 [Brucella abortus]
ATGAAGATCTTCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT79825 WP_002964097.1 NZ_CP022879:1045-1359 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT79825 NZ_CP022879:1045-1359 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: D7H3C9