79823

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 434682..435241 Replicon chromosome
Accession NZ_CP022875
Organism Brucella melitensis strain BL

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag CJP68_RS02215 Protein ID WP_002966807.1
Coordinates 434682..434927 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag CJP68_RS02220 Protein ID WP_004683740.1
Coordinates 434927..435241 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJP68_RS02195 430527..431978 - 1452 WP_006135932.1 hypothetical protein -
CJP68_RS02200 432390..433082 - 693 WP_006135935.1 hypothetical protein -
CJP68_RS02205 433083..433841 - 759 Protein_409 IS5 family transposase -
CJP68_RS02210 433911..434549 - 639 Protein_410 tRNA-guanine transglycosylase -
CJP68_RS02215 434682..434927 + 246 WP_002966807.1 BrnT family toxin Toxin
CJP68_RS02220 434927..435241 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
CJP68_RS02230 435589..435759 + 171 WP_002964095.1 DUF1508 domain-containing protein -
CJP68_RS02235 436107..436817 - 711 WP_004683739.1 porin family protein -
CJP68_RS02240 437163..437639 - 477 WP_002966806.1 hypothetical protein -
CJP68_RS02245 437662..437937 - 276 WP_002964092.1 hypothetical protein -
CJP68_RS02250 437934..438164 - 231 WP_002964091.1 hypothetical protein -
CJP68_RS02255 438161..438865 - 705 WP_005968944.1 hypothetical protein -
CJP68_RS02260 438915..439118 + 204 WP_002964088.1 hypothetical protein -
CJP68_RS02265 439115..439330 + 216 WP_005968947.1 hypothetical protein -
CJP68_RS02270 439333..439536 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbdA / wboA 427413..441308 13895
- flank IS/Tn - - 433473..433841 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T79823 WP_002966807.1 NZ_CP022875:434682-434927 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T79823 NZ_CP022875:434682-434927 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT79823 WP_004683740.1 NZ_CP022875:434927-435241 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT79823 NZ_CP022875:434927-435241 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References