Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 561424..562060 Replicon chromosome
Accession NZ_CP022874
Organism Bacillus sp. 1s-1

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag CJO35_RS02820 Protein ID WP_003179128.1
Coordinates 561710..562060 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag CJO35_RS02815 Protein ID WP_006638778.1
Coordinates 561424..561705 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJO35_RS02795 556539..558020 + 1482 WP_009330132.1 PH domain-containing protein -
CJO35_RS02800 558017..558616 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
CJO35_RS02805 558959..559918 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
CJO35_RS02810 560143..561312 + 1170 WP_026080763.1 alanine racemase -
CJO35_RS02815 561424..561705 + 282 WP_006638778.1 hypothetical protein Antitoxin
CJO35_RS02820 561710..562060 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CJO35_RS02825 562178..563005 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
CJO35_RS02830 563009..563374 + 366 WP_003179132.1 STAS domain-containing protein -
CJO35_RS02835 563377..563778 + 402 WP_003179135.1 anti-sigma regulatory factor -
CJO35_RS02840 563789..564796 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
CJO35_RS02845 564855..565181 + 327 WP_003179140.1 anti-sigma factor antagonist -
CJO35_RS02850 565181..565666 + 486 WP_003179142.1 anti-sigma B factor RsbW -
CJO35_RS02855 565632..566423 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
CJO35_RS02860 566420..567019 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T79821 WP_003179128.1 NZ_CP022874:561710-562060 [Bacillus sp. 1s-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T79821 NZ_CP022874:561710-562060 [Bacillus sp. 1s-1]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT79821 WP_006638778.1 NZ_CP022874:561424-561705 [Bacillus sp. 1s-1]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT79821 NZ_CP022874:561424-561705 [Bacillus sp. 1s-1]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References