79820

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2077151..2077710 Replicon chromosome
Accession NZ_CP022827
Organism Brucella melitensis strain BY38 isolate Qingmin Wu

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag CJJ12_RS10695 Protein ID WP_002966807.1
Coordinates 2077151..2077396 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag CJJ12_RS10700 Protein ID WP_004683740.1
Coordinates 2077396..2077710 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJJ12_RS10680 2074582..2075710 + 1129 WP_011950188.1 peptide chain release factor 2 -
CJJ12_RS10685 2076059..2076340 - 282 WP_006046599.1 IS5/IS1182 family transposase -
CJJ12_RS10690 2076383..2077018 - 636 Protein_1971 tRNA-guanine transglycosylase -
CJJ12_RS10695 2077151..2077396 + 246 WP_002966807.1 BrnT family toxin Toxin
CJJ12_RS10700 2077396..2077710 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
CJJ12_RS10710 2078058..2078228 + 171 WP_002964095.1 DUF1508 domain-containing protein -
CJJ12_RS10715 2078576..2079286 - 711 WP_004683739.1 porin family protein -
CJJ12_RS10720 2079632..2080108 - 477 WP_002966806.1 hypothetical protein -
CJJ12_RS10725 2080131..2080406 - 276 WP_002964092.1 hypothetical protein -
CJJ12_RS10730 2080403..2080633 - 231 WP_002964091.1 hypothetical protein -
CJJ12_RS10735 2080630..2081334 - 705 WP_005968944.1 hypothetical protein -
CJJ12_RS10740 2081384..2081587 + 204 WP_002964088.1 hypothetical protein -
CJJ12_RS10745 2081584..2081799 + 216 WP_005968947.1 hypothetical protein -
CJJ12_RS10750 2081802..2082005 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2076059..2076331 272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T79820 WP_002966807.1 NZ_CP022827:2077151-2077396 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T79820 NZ_CP022827:2077151-2077396 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT79820 WP_004683740.1 NZ_CP022827:2077396-2077710 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT79820 NZ_CP022827:2077396-2077710 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References