Detailed information of TA system TA079818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5457522..5458138
• Replicon: chromosome
• Accession: NZ_CP022823
• Organism: Klebsiella quasivariicola strain KPN1705

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A223UJ29
• Locus tag: B8P98_RS27435
• Protein ID: WP_025710915.1
• Coordinates: 5457522..5457896 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B8P98_RS27440
• Protein ID: WP_025710914.1
• Coordinates: 5457896..5458138 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8P98_RS27420	5455024..5455926	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
B8P98_RS27425	5455923..5456558	+	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
B8P98_RS27430	5456555..5457484	+	930	WP_025710916.1	formate dehydrogenase accessory protein FdhE	-
B8P98_RS27435	5457522..5457896	-	375	WP_025710915.1	type II toxin-antitoxin system VapC family toxin	Toxin
B8P98_RS27440	5457896..5458138	-	243	WP_025710914.1	ribbon-helix-helix protein, CopG family	Antitoxin
B8P98_RS27445	5458343..5459260	+	918	WP_095033595.1	alpha/beta hydrolase	-
B8P98_RS27450	5459274..5460215	-	942	WP_025710912.1	fatty acid biosynthesis protein FabY	-
B8P98_RS27455	5460260..5460697	-	438	WP_004203627.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B8P98_RS27460	5460694..5461554	-	861	WP_025710911.1	virulence factor BrkB family protein	-
B8P98_RS27465	5461548..5462147	-	600	WP_095033596.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14177.42 Da
• Isoelectric Point: 7.4021

>T79818 WP_025710915.1 NZ_CP022823:c5457896-5457522 [Klebsiella quasivariicola]
MVKGVALFDTNILIDLFSGRREAKEALESWPSQNAISLITWMEVMVGAKKYHQEQRTRIALSSFNIIDVSHEIAERSVDL
RHEYRMKLPDAIILATARVHRLELITRNTKDFAGIPGVLTPYEL

Nucleotide

• Length: 375 bp

>T79818 NZ_CP022823:c5457896-5457522 [Klebsiella quasivariicola]
ATGGTTAAGGGCGTCGCGCTATTTGATACTAATATTCTCATTGATTTATTTAGCGGGCGGCGTGAAGCCAAGGAGGCGTT
AGAGTCCTGGCCGTCGCAGAATGCAATTAGTTTAATTACCTGGATGGAGGTCATGGTTGGCGCCAAAAAGTATCACCAGG
AGCAGCGTACGCGGATTGCCTTGAGCTCTTTTAACATCATTGATGTTTCGCATGAGATTGCTGAACGAAGCGTCGATCTG
CGACATGAGTATCGCATGAAACTGCCGGACGCCATTATCCTGGCGACGGCGCGGGTTCATCGTCTTGAACTGATTACACG
GAATACGAAGGATTTTGCAGGCATTCCCGGCGTGCTCACACCGTATGAATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9343.57 Da
• Isoelectric Point: 4.3713

>AT79818 WP_025710914.1 NZ_CP022823:c5458138-5457896 [Klebsiella quasivariicola]
MLAESDMGRILLDLPDDVIKRLDDLKAQRNLPRAELLREAVEQYLEKQDRAKESISGALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT79818 NZ_CP022823:c5458138-5457896 [Klebsiella quasivariicola]
ATGCTGGCTGAAAGCGATATGGGCAGAATTCTTCTCGATTTACCAGACGATGTCATCAAACGGCTGGATGACCTCAAAGC
GCAGCGTAATCTTCCGCGAGCGGAGCTGTTACGTGAAGCCGTTGAACAATATCTGGAGAAGCAGGATCGGGCTAAAGAGA
GCATTTCAGGCGCCCTCGGCCTGTGGCAAGGCTGCGAAGAAGACGGCGTTGAATACCAACGTAAACTGCGCGAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A223UJ29