Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4217523..4218142 Replicon chromosome
Accession NZ_CP022823
Organism Klebsiella quasivariicola strain KPN1705

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B8P98_RS21355 Protein ID WP_002892050.1
Coordinates 4217924..4218142 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A223UF87
Locus tag B8P98_RS21350 Protein ID WP_025710692.1
Coordinates 4217523..4217897 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8P98_RS21340 4212676..4213869 + 1194 WP_025710694.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B8P98_RS21345 4213892..4217038 + 3147 WP_025710693.1 multidrug efflux RND transporter permease subunit -
B8P98_RS21350 4217523..4217897 + 375 WP_025710692.1 Hha toxicity modulator TomB Antitoxin
B8P98_RS21355 4217924..4218142 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
B8P98_RS21360 4218300..4218866 + 567 WP_025710691.1 maltose O-acetyltransferase -
B8P98_RS31150 4218838..4218966 - 129 Protein_4090 hypothetical protein -
B8P98_RS21365 4219003..4219473 + 471 WP_025710690.1 YlaC family protein -
B8P98_RS21370 4219448..4220899 - 1452 WP_080924892.1 PLP-dependent aminotransferase family protein -
B8P98_RS21375 4221000..4221698 + 699 WP_025710688.1 GNAT family N-acetyltransferase -
B8P98_RS21380 4221695..4221835 - 141 WP_025710687.1 type B 50S ribosomal protein L36 -
B8P98_RS21385 4221835..4222098 - 264 WP_080924893.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T79815 WP_002892050.1 NZ_CP022823:4217924-4218142 [Klebsiella quasivariicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T79815 NZ_CP022823:4217924-4218142 [Klebsiella quasivariicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATCGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTCTACGATAAGATCCCGACGTCGGTCTGGAAATTTATCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14398.04 Da        Isoelectric Point: 4.7295

>AT79815 WP_025710692.1 NZ_CP022823:4217523-4217897 [Klebsiella quasivariicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIDQVDEYL
DDTFTLFSNYGINSSDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT79815 NZ_CP022823:4217523-4217897 [Klebsiella quasivariicola]
ATGGACGAATACTCGCCAAAAAGACATGATATAGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGGTGGGTTAACGACCCGACATCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCCGAGGATAATAAACTGATCGATCAAGTTGACGAATATCTG
GACGATACTTTCACATTGTTCAGTAACTACGGCATTAACTCCTCGGATTTGCAGAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A223UF87

References