Detailed information of TA system TA079805

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 874940..875640
• Replicon: chromosome
• Accession: NZ_CP022823
• Organism: Klebsiella quasivariicola strain KPN1705

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7H4NX47
• Locus tag: B8P98_RS04440
• Protein ID: WP_001546951.1
• Coordinates: 875317..875640 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B8P98_RS04435
• Protein ID: WP_095032769.1
• Coordinates: 874940..875296 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8P98_RS04405	870611..871291	-	681	WP_095032767.1	heavy metal response regulator transcription factor	-
B8P98_RS04410	871306..872472	-	1167	WP_174705283.1	aldo/keto reductase	-
B8P98_RS04415	872724..873773	+	1050	WP_095032768.1	alpha/beta hydrolase	-
B8P98_RS04420	874030..874365	+	336	Protein_839	nitroreductase family protein	-
B8P98_RS04425	874364..874696	+	333	Protein_840	DNA repair protein RadC	-
B8P98_RS04430	874693..874914	+	222	WP_014226747.1	DUF987 domain-containing protein	-
B8P98_RS04435	874940..875296	+	357	WP_095032769.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B8P98_RS04440	875317..875640	+	324	WP_001546951.1	TA system toxin CbtA family protein	Toxin
B8P98_RS04445	876012..877400	-	1389	WP_095032770.1	MFS transporter	-
B8P98_RS04450	877530..878444	+	915	WP_025713603.1	LysR family transcriptional regulator	-
B8P98_RS04455	878748..879551	-	804	WP_095032771.1	carboxymuconolactone decarboxylase family protein	-
B8P98_RS04460	879584..880603	-	1020	WP_095032772.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12496.55 Da
• Isoelectric Point: 7.1331

>T79805 WP_001546951.1 NZ_CP022823:875317-875640 [Klebsiella quasivariicola]
MKFLPTTNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T79805 NZ_CP022823:875317-875640 [Klebsiella quasivariicola]
ATGAAATTTCTACCTACAACAAATATGCGGGCGGCGAAGCCATGCCTGTCGCCCGTGACTATCTGGCAAATGCTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACTCCATATCTCACGATTATTGATATCATGAGGGCCCGTCGCGATCTGGGTTTAATGAATCGTAA
TTAG

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13187.86 Da
• Isoelectric Point: 6.2009

>AT79805 WP_095032769.1 NZ_CP022823:874940-875296 [Klebsiella quasivariicola]
MNNHSESGTKPENPRCQHWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFCDDDALYLDQAFPLILKQLELILTGGE
LSPRHQHCVTLYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 357 bp

>AT79805 NZ_CP022823:874940-875296 [Klebsiella quasivariicola]
ATGAACAATCACTCAGAATCCGGTACCAAGCCGGAGAATCCCCGCTGCCAGCATTGGGGGCTAAAGAGTACGATTACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTTGCTGATCGGGCCGGATTTAACGGTGCTTTCT
GCGACGATGACGCGCTATACCTGGATCAGGCCTTTCCGTTGATTCTCAAACAACTGGAGCTGATACTCACCGGCGGAGAA
CTCTCCCCCCGCCATCAGCACTGTGTCACCCTCTATCACAACGGATTCACCTGCGAAGCCGATACGCTTGGTAGTTGTGG
CTACGTATACATCGCCATTTATCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A7H4NX47