Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 281865..282496 Replicon chromosome
Accession NZ_CP022823
Organism Klebsiella quasivariicola strain KPN1705

Toxin (Protein)


Gene name hicA Uniprot ID A0A223U5E0
Locus tag B8P98_RS01340 Protein ID WP_025710732.1
Coordinates 281865..282140 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag B8P98_RS01345 Protein ID WP_004181489.1
Coordinates 282137..282496 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8P98_RS01320 277102..277437 + 336 WP_002921203.1 universal stress protein UspB -
B8P98_RS01325 277496..278992 - 1497 WP_025710729.1 inorganic phosphate transporter PitA -
B8P98_RS01330 279222..280415 + 1194 WP_025710730.1 NAD(P)/FAD-dependent oxidoreductase -
B8P98_RS01335 280455..281468 - 1014 WP_025710731.1 magnesium transporter -
B8P98_RS01340 281865..282140 + 276 WP_025710732.1 type II toxin-antitoxin system HicA family toxin Toxin
B8P98_RS01345 282137..282496 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B8P98_RS01350 282524..282925 - 402 WP_004181488.1 nickel-responsive transcriptional regulator NikR -
B8P98_RS01355 282913..283704 - 792 WP_080897539.1 nickel import ATP-binding protein NikE -
B8P98_RS01360 283701..284465 - 765 WP_080897538.1 nickel import ATP-binding protein NikD -
B8P98_RS01365 284465..285298 - 834 WP_025710735.1 nickel ABC transporter permease subunit NikC -
B8P98_RS01370 285295..286239 - 945 WP_023285608.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10266.91 Da        Isoelectric Point: 10.6867

>T79802 WP_025710732.1 NZ_CP022823:281865-282140 [Klebsiella quasivariicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKKGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T79802 NZ_CP022823:281865-282140 [Klebsiella quasivariicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGTTGGAGCAACTGTTTAAAACCCCTGTCCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAAAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACCGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT79802 WP_004181489.1 NZ_CP022823:282137-282496 [Klebsiella quasivariicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT79802 NZ_CP022823:282137-282496 [Klebsiella quasivariicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTCGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A223U5E0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References