Detailed information of TA system TA079753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 63935..64545
• Replicon: plasmid unnamed
• Accession: NZ_CP022775
• Organism: Ralstonia solanacearum strain T12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CJO85_RS16785
• Protein ID: WP_075465902.1
• Coordinates: 64237..64545 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CJO85_RS16780
• Protein ID: WP_013209163.1
• Coordinates: 63935..64240 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO85_RS16740 (CJO85_16630)	59099..59509	+	411	WP_075465861.1	thioesterase family protein	-
CJO85_RS16745 (CJO85_16635)	59547..59938	+	392	Protein_52	RidA family protein	-
CJO85_RS16750 (CJO85_16640)	60254..60535	-	282	WP_075465894.1	hypothetical protein	-
CJO85_RS16755 (CJO85_16645)	60668..60952	-	285	WP_075465896.1	hypothetical protein	-
CJO85_RS16765 (CJO85_16650)	61286..61588	+	303	Protein_55	transposase	-
CJO85_RS25335	61950..62306	+	357	WP_246761104.1	hypothetical protein	-
CJO85_RS16775 (CJO85_16660)	62411..63724	-	1314	WP_118884159.1	MFS family transporter	-
CJO85_RS16780 (CJO85_16665)	63935..64240	-	306	WP_013209163.1	putative addiction module antidote protein	Antitoxin
CJO85_RS16785 (CJO85_16670)	64237..64545	-	309	WP_075465902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CJO85_RS16790 (CJO85_16675)	64630..65682	-	1053	WP_246761202.1	MFS transporter	-
CJO85_RS16795 (CJO85_16680)	65955..66770	-	816	WP_075465905.1	GntR family transcriptional regulator	-
CJO85_RS16800 (CJO85_16685)	66944..67498	-	555	WP_118884160.1	helix-turn-helix domain-containing protein	-
CJO85_RS16805 (CJO85_16690)	67630..68226	+	597	WP_118884161.1	GNAT family N-acetyltransferase	-
CJO85_RS16810 (CJO85_16695)	68482..68916	+	435	WP_013209169.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1988373	1988373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11795.54 Da
• Isoelectric Point: 8.5237

>T79753 WP_075465902.1 NZ_CP022775:c64545-64237 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRVQVRIDRLQMGNPGDVKAVREGIREMRIDHGPGYRVYFSQRGVVLIVLLCGGDKS
TQEADVRRAIDLSRRLDVDNME

Nucleotide

• Length: 309 bp

>T79753 NZ_CP022775:c64545-64237 [Ralstonia solanacearum]
ATGCTGACAATCCGCACGACTGAAATCTTCGACGACTGGTTTTGCAGTCTCCGCGACAAGGCCGCGCAACGTCGCGTCCA
GGTGCGCATCGATCGCCTGCAGATGGGCAATCCAGGCGACGTGAAAGCTGTACGTGAAGGCATCCGTGAGATGCGGATCG
ATCATGGCCCGGGCTATCGCGTGTATTTTTCACAGCGTGGCGTGGTCCTGATCGTCCTGCTGTGCGGCGGTGACAAGTCG
ACGCAGGAAGCCGATGTCCGCCGCGCAATCGACCTGTCCCGCCGTCTTGACGTGGACAACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11009.67 Da
• Isoelectric Point: 6.7174

>AT79753 WP_013209163.1 NZ_CP022775:c64240-63935 [Ralstonia solanacearum]
MMMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARARGMTQIARETGMTREGLYKALSEDGNP
SFATVLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT79753 NZ_CP022775:c64240-63935 [Ralstonia solanacearum]
ATGATGATGGCCCAACGCACCATAAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTATCTCGACGCCTGCCTGGAGGAAGCCGGGGATGACCCCGCATTCATCACGCACGCGCTGGGCGTGGTCGCGCGGGCGC
GCGGCATGACGCAGATCGCCCGCGAGACCGGGATGACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA