Detailed information of TA system TA079712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 510036..510706
• Replicon: chromosome
• Accession: NZ_CP022752
• Organism: Actinopolyspora erythraea strain YIM 90600

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A099D1V0
• Locus tag: CDG81_RS02320
• Protein ID: WP_043576097.1
• Coordinates: 510272..510706 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDG81_RS02315
• Protein ID: WP_084134234.1
• Coordinates: 510036..510275 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG81_RS02300	505371..506580	-	1210	Protein_437	ABC transporter permease	-
CDG81_RS02305	506722..508695	-	1974	WP_043576102.1	FUSC family protein	-
CDG81_RS02310	508949..509827	-	879	WP_043576099.1	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-
CDG81_RS02315	510036..510275	+	240	WP_084134234.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDG81_RS02320	510272..510706	+	435	WP_043576097.1	PIN domain-containing protein	Toxin
CDG81_RS02325	511040..512695	-	1656	WP_052428398.1	phosphomethylpyrimidine synthase ThiC	-
CDG81_RS02330	513058..513549	+	492	WP_198319417.1	Uma2 family endonuclease	-
CDG81_RS02335	513645..514220	-	576	WP_043576094.1	peptide deformylase	-
CDG81_RS02340	514502..514972	+	471	WP_043576091.1	DUF3263 domain-containing protein	-
CDG81_RS02345	514973..515650	+	678	WP_043576089.1	LytR C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (4-127)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-40)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15970.88 Da
• Isoelectric Point: 6.0969

>T79712 WP_043576097.1 NZ_CP022752:510272-510706 [Actinopolyspora erythraea]
VKRALLDVNVLLALLDSDHVDHERAHEWLDADGTAGWASCSITENGFVRIISQPRYPSPISTTEAVELLARTRHESNHEF
WACTVSLLDPHSVDRTRLHGHRQITDAYLLALATAHDGRLVTLDNSLPLSAAHSATEEHLTVLK

Nucleotide

• Length: 435 bp

>T79712 NZ_CP022752:510272-510706 [Actinopolyspora erythraea]
GTGAAACGAGCCCTGCTCGATGTGAATGTGCTGCTGGCCCTGCTGGACAGCGACCATGTCGACCATGAGCGTGCACACGA
ATGGCTGGACGCCGACGGCACAGCCGGGTGGGCCTCGTGCTCTATCACCGAGAACGGCTTCGTTCGCATCATCAGTCAAC
CGCGCTACCCCAGCCCCATTTCCACAACCGAGGCCGTCGAGTTGCTTGCCCGCACACGTCATGAAAGCAACCATGAGTTC
TGGGCGTGCACGGTCAGTCTGCTGGATCCGCACTCGGTGGACCGCACGCGGTTGCACGGTCACCGTCAGATAACCGATGC
CTACCTGCTGGCCTTGGCCACGGCACACGACGGACGCCTGGTTACCTTGGACAACTCGCTTCCGCTCTCCGCTGCGCACA
GCGCTACTGAGGAGCACCTGACTGTTCTGAAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8909.93 Da
• Isoelectric Point: 7.4735

>AT79712 WP_084134234.1 NZ_CP022752:510036-510275 [Actinopolyspora erythraea]
MRTTIRLDDDVLAAVHERARREKRSAGQVLSELARQALTRSGHPTEPRGASHHGFRPLPHRGPAVSNELIDQIREEEPE

Nucleotide

• Length: 240 bp

>AT79712 NZ_CP022752:510036-510275 [Actinopolyspora erythraea]
ATGCGCACTACGATCCGGCTGGACGATGACGTTCTGGCCGCCGTCCACGAACGGGCTCGTCGTGAGAAGCGTTCGGCCGG
GCAGGTGCTGTCCGAACTCGCTCGTCAGGCACTGACTCGTTCCGGGCACCCGACCGAGCCACGGGGTGCCTCGCATCACG
GATTTCGGCCATTACCCCACCGAGGGCCCGCCGTGTCGAACGAGCTCATCGATCAGATCCGCGAGGAAGAGCCAGAGTGA

Structures

Toxin

• AlphaFold DB: A0A099D1V0