Detailed information of TA system TA079691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 56801..57402
• Replicon: plasmid pSA186_4
• Accession: NZ_CP022733
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: CHQ92_RS25640
• Protein ID: WP_001216045.1
• Coordinates: 56801..57181 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CHQ92_RS25645
• Protein ID: WP_001190712.1
• Coordinates: 57181..57402 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS25615	52242..53726	-	1485	WP_000124159.1	hypothetical protein	-
CHQ92_RS25620	53726..54919	-	1194	WP_000219618.1	hypothetical protein	-
CHQ92_RS25625	55005..55457	-	453	WP_001326849.1	late promoter-activating protein	-
CHQ92_RS25630	55546..56589	-	1044	WP_000648833.1	DUF968 domain-containing protein	-
CHQ92_RS25635	56617..56796	-	180	WP_001339207.1	hypothetical protein	-
CHQ92_RS25640	56801..57181	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CHQ92_RS25645	57181..57402	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CHQ92_RS25650	57475..57864	-	390	WP_000506726.1	DNA repair protein	-
CHQ92_RS25655	57988..58239	-	252	WP_001283837.1	DNA polymerase III subunit theta	-
CHQ92_RS25660	58413..58622	+	210	WP_001344848.1	hypothetical protein	-
CHQ92_RS25665	58607..58891	-	285	WP_023154374.1	hypothetical protein	-
CHQ92_RS25670	58875..59813	-	939	WP_023154375.1	hypothetical protein	-
CHQ92_RS25675	59795..60169	-	375	WP_023154376.1	hypothetical protein	-
CHQ92_RS25680	60176..60469	-	294	WP_000269004.1	hypothetical protein	-
CHQ92_RS25685	60648..60881	-	234	WP_000517421.1	hypothetical protein	-
CHQ92_RS25690	60958..61218	-	261	WP_089590754.1	eaa protein	-
CHQ92_RS25695	61215..62096	-	882	WP_089590755.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96658	96658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T79691 WP_001216045.1 NZ_CP022733:c57181-56801 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T79691 NZ_CP022733:c57181-56801 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT79691 WP_001190712.1 NZ_CP022733:c57402-57181 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT79691 NZ_CP022733:c57402-57181 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6