Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 152961..153486 Replicon plasmid pSA186_2
Accession NZ_CP022731
Organism Escherichia coli strain SA186

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CHQ92_RS24370 Protein ID WP_001159871.1
Coordinates 153181..153486 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CHQ92_RS24365 Protein ID WP_000813630.1
Coordinates 152961..153179 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHQ92_RS24330 148460..148771 - 312 Protein_142 type II toxin-antitoxin system VapC family toxin -
CHQ92_RS24335 148768..148998 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CHQ92_RS24340 149263..149763 + 501 WP_000528932.1 hypothetical protein -
CHQ92_RS24345 149776..150549 + 774 WP_000905949.1 hypothetical protein -
CHQ92_RS24350 150716..151849 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
CHQ92_RS24355 151883..152395 - 513 WP_000151784.1 hypothetical protein -
CHQ92_RS24365 152961..153179 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CHQ92_RS24370 153181..153486 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CHQ92_RS24375 153487..154293 + 807 WP_000016968.1 site-specific integrase -
CHQ92_RS24380 154973..155704 - 732 WP_000504262.1 replication initiation protein -
CHQ92_RS24385 156325..157530 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..198748 198748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T79682 WP_001159871.1 NZ_CP022731:153181-153486 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T79682 NZ_CP022731:153181-153486 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT79682 WP_000813630.1 NZ_CP022731:152961-153179 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT79682 NZ_CP022731:152961-153179 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References