Detailed information of TA system TA079680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 10110..10645
• Replicon: plasmid pSA186_2
• Accession: NZ_CP022731
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: CHQ92_RS23580
• Protein ID: WP_000222760.1
• Coordinates: 10358..10645 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: CHQ92_RS23575
• Protein ID: WP_001132900.1
• Coordinates: 10110..10361 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS23530	5237..5797	+	561	WP_000139315.1	fertility inhibition protein FinO	-
CHQ92_RS23535	5933..6145	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CHQ92_RS28225	6391..6465	+	75	Protein_5	endonuclease	-
-	6721..6782	-	62	NuclAT_1	-	-
-	6721..6782	-	62	NuclAT_1	-	-
-	6721..6782	-	62	NuclAT_1	-	-
-	6721..6782	-	62	NuclAT_1	-	-
CHQ92_RS23545	6769..6975	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
CHQ92_RS23550	7259..7516	+	258	WP_000083845.1	replication regulatory protein RepA	-
CHQ92_RS23560	7748..7822	+	75	WP_001442103.1	RepA leader peptide Tap	-
CHQ92_RS23565	7815..8297	+	483	WP_001273588.1	hypothetical protein	-
CHQ92_RS23570	8290..9147	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
CHQ92_RS23575	10110..10361	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CHQ92_RS23580	10358..10645	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHQ92_RS23590	11085..11432	-	348	Protein_13	IS1-like element IS1A family transposase	-
CHQ92_RS23600	11521..12543	+	1023	WP_129547715.1	IS21 family transposase	-
CHQ92_RS23605	12540..13322	+	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
CHQ92_RS23610	13377..13741	-	365	Protein_16	IS1 family transposase	-
CHQ92_RS23615	13841..15062	+	1222	Protein_17	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..198748	198748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T79680 WP_000222760.1 NZ_CP022731:10358-10645 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T79680 NZ_CP022731:10358-10645 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT79680 WP_001132900.1 NZ_CP022731:10110-10361 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT79680 NZ_CP022731:10110-10361 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2