Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3388005..3388525 Replicon chromosome
Accession NZ_CP022730
Organism Escherichia coli strain SA186

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag CHQ92_RS16505 Protein ID WP_001160964.1
Coordinates 3388241..3388525 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CHQ92_RS16500 Protein ID WP_000125566.1
Coordinates 3388005..3388238 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHQ92_RS16475 3383078..3383365 + 288 WP_063268564.1 ferredoxin-like protein FixX -
CHQ92_RS16480 3383424..3384755 + 1332 WP_001183209.1 MFS transporter -
CHQ92_RS16485 3384863..3385393 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CHQ92_RS16490 3385386..3387248 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
CHQ92_RS16495 3387440..3387919 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
CHQ92_RS16500 3388005..3388238 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CHQ92_RS16505 3388241..3388525 + 285 WP_001160964.1 CcdB family protein Toxin
CHQ92_RS16510 3388552..3389400 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CHQ92_RS16515 3389407..3389784 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CHQ92_RS16520 3389787..3390608 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CHQ92_RS16525 3390605..3391594 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CHQ92_RS16530 3391594..3392880 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T79673 WP_001160964.1 NZ_CP022730:3388241-3388525 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T79673 NZ_CP022730:3388241-3388525 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT79673 WP_000125566.1 NZ_CP022730:3388005-3388238 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT79673 NZ_CP022730:3388005-3388238 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References