Detailed information of TA system TA079672

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3200394..3201095
• Replicon: chromosome
• Accession: NZ_CP022730
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: CHQ92_RS15645
• Protein ID: WP_001531265.1
• Coordinates: 3200760..3201095 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CHQ92_RS15640
• Protein ID: WP_032143152.1
• Coordinates: 3200394..3200729 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS15615	3196341..3196559	-	219	WP_001531256.1	AlpA family phage regulatory protein	-
CHQ92_RS15620	3196677..3197291	-	615	WP_001531257.1	inovirus Gp2 family protein	-
CHQ92_RS15625	3197884..3198837	+	954	WP_001531259.1	hypothetical protein	-
CHQ92_RS15630	3199073..3199891	+	819	WP_001531260.1	DUF945 domain-containing protein	-
CHQ92_RS15635	3199921..3200394	+	474	WP_001531261.1	DNA repair protein RadC	-
CHQ92_RS15640	3200394..3200729	+	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CHQ92_RS15645	3200760..3201095	+	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
CHQ92_RS15650	3201226..3202059	+	834	WP_001531266.1	DUF4942 domain-containing protein	-
CHQ92_RS15655	3202136..3202384	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
CHQ92_RS15660	3202388..3202663	+	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CHQ92_RS15665	3202777..3203574	+	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
CHQ92_RS15675	3203985..3204156	+	172	Protein_2990	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T79672 WP_001531265.1 NZ_CP022730:3200760-3201095 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T79672 NZ_CP022730:3200760-3201095 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT79672 WP_032143152.1 NZ_CP022730:3200394-3200729 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT79672 NZ_CP022730:3200394-3200729 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3