Detailed information of TA system TA079633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2059944..2060473
• Replicon: chromosome
• Accession: NZ_CP022718
• Organism: Staphylococcus aureus strain 45

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CGP85_RS10550
• Protein ID: WP_000621175.1
• Coordinates: 2059944..2060306 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CGP85_RS10555
• Protein ID: WP_000948331.1
• Coordinates: 2060303..2060473 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGP85_RS10525	2056923..2057693	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CGP85_RS10530	2057668..2058147	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CGP85_RS10535	2058149..2058475	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CGP85_RS10540	2058594..2059595	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CGP85_RS10550	2059944..2060306	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CGP85_RS10555	2060303..2060473	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CGP85_RS10560	2060558..2061706	-	1149	WP_000130547.1	alanine racemase	-
CGP85_RS10565	2061772..2062131	-	360	WP_000581202.1	holo-ACP synthase	-
CGP85_RS10570	2062135..2062626	-	492	WP_001205912.1	PH domain-containing protein	-
CGP85_RS10575	2062613..2064196	-	1584	WP_001294620.1	PH domain-containing protein	-
CGP85_RS10580	2064189..2064668	-	480	WP_001287079.1	hypothetical protein	-
CGP85_RS10585	2064877..2065437	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T79633 WP_000621175.1 NZ_CP022718:c2060306-2059944 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T79633 NZ_CP022718:c2060306-2059944 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT79633 WP_000948331.1 NZ_CP022718:c2060473-2060303 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT79633 NZ_CP022718:c2060473-2060303 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1