Detailed information of TA system TA079624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2161771..2162285
• Replicon: chromosome
• Accession: NZ_CP022717
• Organism: Staphylococcus aureus strain 27

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: CGP83_RS11140
• Protein ID: WP_000581792.1
• Coordinates: 2161771..2161980 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: CGP83_RS11145
• Protein ID: WP_000138932.1
• Coordinates: 2161992..2162285 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGP83_RS11120	2157279..2158637	-	1359	WP_000611461.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
CGP83_RS11125	2158652..2159722	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
CGP83_RS11130	2160040..2161242	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
CGP83_RS11135	2161506..2161643	-	138	WP_000828354.1	hypothetical protein	-
CGP83_RS11140	2161771..2161980	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
CGP83_RS11145	2161992..2162285	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
CGP83_RS11150	2162451..2163935	+	1485	WP_000571551.1	cardiolipin synthase	-
CGP83_RS11155	2163963..2164610	+	648	WP_001187620.1	HD domain-containing protein	-
CGP83_RS11170	2165241..2166113	-	873	WP_000725802.1	membrane protein insertase YidC	-
CGP83_RS11175	2166199..2166840	-	642	WP_000483158.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T79624 WP_000581792.1 NZ_CP022717:c2161980-2161771 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T79624 NZ_CP022717:c2161980-2161771 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACGCCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT79624 WP_000138932.1 NZ_CP022717:c2162285-2161992 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT79624 NZ_CP022717:c2162285-2161992 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAAGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13