Detailed information of TA system TA079623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2142027..2142556
• Replicon: chromosome
• Accession: NZ_CP022717
• Organism: Staphylococcus aureus strain 27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CGP83_RS11050
• Protein ID: WP_000621175.1
• Coordinates: 2142027..2142389 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CGP83_RS11055
• Protein ID: WP_000948331.1
• Coordinates: 2142386..2142556 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGP83_RS11025	2139006..2139776	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CGP83_RS11030	2139751..2140230	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CGP83_RS11035	2140232..2140558	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CGP83_RS11040	2140677..2141678	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CGP83_RS11050	2142027..2142389	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CGP83_RS11055	2142386..2142556	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CGP83_RS11060	2142641..2143789	-	1149	WP_001281143.1	alanine racemase	-
CGP83_RS11065	2143855..2144214	-	360	WP_000581198.1	holo-ACP synthase	-
CGP83_RS11070	2144218..2144709	-	492	WP_001205919.1	PH domain-containing protein	-
CGP83_RS11075	2144696..2146279	-	1584	WP_001294640.1	PH domain-containing protein	-
CGP83_RS11080	2146272..2146751	-	480	WP_001287075.1	hypothetical protein	-
CGP83_RS11085	2146960..2147520	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T79623 WP_000621175.1 NZ_CP022717:c2142389-2142027 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T79623 NZ_CP022717:c2142389-2142027 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT79623 WP_000948331.1 NZ_CP022717:c2142556-2142386 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT79623 NZ_CP022717:c2142556-2142386 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1