Detailed information of TA system TA079613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 816072..816700
• Replicon: chromosome
• Accession: NZ_CP022715
• Organism: Actinobacillus pleuropneumoniae strain KL 16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CHY23_RS04030
• Protein ID: WP_005599242.1
• Coordinates: 816302..816700 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A828PGB0
• Locus tag: CHY23_RS04025
• Protein ID: WP_005599241.1
• Coordinates: 816072..816302 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHY23_RS04020	814045..815631	+	1587	WP_005599240.1	DUF262 domain-containing protein	-
CHY23_RS04025	816072..816302	+	231	WP_005599241.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CHY23_RS04030	816302..816700	+	399	WP_005599242.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CHY23_RS04035	816835..817251	-	417	WP_005599244.1	DUF417 family protein	-
CHY23_RS04040	817488..818678	-	1191	WP_005599245.1	pentapeptide repeat-containing protein	-
CHY23_RS04045	818731..820053	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
CHY23_RS04050	820199..820720	-	522	WP_005599247.1	ATP-dependent protease subunit HslV	-
CHY23_RS04055	820798..821511	-	714	WP_005609162.1	DNA/RNA nuclease SfsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	800694..818678	17984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15028.31 Da
• Isoelectric Point: 7.4716

>T79613 WP_005599242.1 NZ_CP022715:816302-816700 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPLEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPEQNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKHGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLANWV

Nucleotide

• Length: 399 bp

>T79613 NZ_CP022715:816302-816700 [Actinobacillus pleuropneumoniae]
ATGCTGACTTATATGCTTGATACTAACATTGCGATTTATGTCATTAAACGCAGACCACTTGAAGTGCTGGATAAGTTTAA
TTTAAATTCCACCCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGGGGCAGAGAAAAGCCAGTTTCCGG
AACAGAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCACGGTAAGATTATCGGGGAGAACGATATTCACATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTACGATTAGCTAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8561.71 Da
• Isoelectric Point: 4.7063

>AT79613 WP_005599241.1 NZ_CP022715:816072-816302 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISIQPEREGL

Nucleotide

• Length: 231 bp

>AT79613 NZ_CP022715:816072-816302 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCGGTATTTATGACAAACCGTAGTCAAGCGGTACGATTGCCGGCAGAAGTACGCTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTCGGTAATGAGCGTATTTTGGCTCCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCATTCAGCCCGAACGTGAGGGACTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828PGB0