Detailed information of TA system TA079612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 602256..602987
• Replicon: chromosome
• Accession: NZ_CP022715
• Organism: Actinobacillus pleuropneumoniae strain KL 16

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CHY23_RS02950
• Protein ID: WP_005598840.1
• Coordinates: 602256..602723 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: CHY23_RS02955
• Protein ID: WP_005619269.1
• Coordinates: 602727..602987 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHY23_RS02925	597618..597989	-	372	WP_005598830.1	CrcB family protein	-
CHY23_RS02930	597992..599053	-	1062	Protein_555	MFS transporter	-
CHY23_RS02935	599148..600257	+	1110	WP_005598834.1	anhydro-N-acetylmuramic acid kinase	-
CHY23_RS02940	600311..601225	+	915	WP_005598836.1	N-acetylmuramic acid 6-phosphate etherase	-
CHY23_RS02945	601292..602173	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
CHY23_RS02950	602256..602723	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
CHY23_RS02955	602727..602987	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
CHY23_RS02960	603108..604568	-	1461	WP_005598844.1	metalloprotease TldD	-
CHY23_RS02965	604698..605957	+	1260	WP_005598846.1	tRNA lysidine(34) synthetase TilS	-
CHY23_RS02970	605984..606274	-	291	WP_005598848.1	DUF5389 family protein	-
CHY23_RS02975	606276..607169	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T79612 WP_005598840.1 NZ_CP022715:c602723-602256 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T79612 NZ_CP022715:c602723-602256 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT79612 WP_005619269.1 NZ_CP022715:c602987-602727 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT79612 NZ_CP022715:c602987-602727 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7