Detailed information of TA system TA079608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 68107..68786
• Replicon: plasmid pAtBDGP1A
• Accession: NZ_CP022700
• Organism: Acetobacter tropicalis strain BDGP1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CIW82_RS18505
• Protein ID: WP_086897721.1
• Coordinates: 68376..68786 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CIW82_RS18500
• Protein ID: WP_176373091.1
• Coordinates: 68107..68379 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW82_RS18465	63802..64149	-	348	WP_086897728.1	hypothetical protein	-
CIW82_RS18470	64734..65414	+	681	WP_086897727.1	hypothetical protein	-
CIW82_RS18475	65474..65773	+	300	WP_086897726.1	hypothetical protein	-
CIW82_RS19580	65766..65912	+	147	WP_162870764.1	hypothetical protein	-
CIW82_RS18485	66636..67124	+	489	WP_086897724.1	single-stranded DNA-binding protein	-
CIW82_RS18490	67187..67621	+	435	WP_086897723.1	plasmid protein	-
CIW82_RS18495	67618..68034	+	417	WP_086897722.1	hypothetical protein	-
CIW82_RS18500	68107..68379	+	273	WP_176373091.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CIW82_RS18505	68376..68786	+	411	WP_086897721.1	type II toxin-antitoxin system VapC family toxin	Toxin
CIW82_RS18510	68942..69676	+	735	WP_086897720.1	CPBP family intramembrane metalloprotease	-
CIW82_RS18520	70225..70437	+	213	WP_086651131.1	hypothetical protein	-
CIW82_RS18525	70502..70681	-	180	WP_086897718.1	hypothetical protein	-
CIW82_RS18530	70688..71080	-	393	WP_116100434.1	hypothetical protein	-
CIW82_RS18535	71099..71911	-	813	WP_086897716.1	hypothetical protein	-
CIW82_RS18540	72008..72472	-	465	WP_086897715.1	hypothetical protein	-
CIW82_RS18545	72475..73323	-	849	WP_058988182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..151013	151013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14829.17 Da
• Isoelectric Point: 5.2057

>T79608 WP_086897721.1 NZ_CP022700:68376-68786 [Acetobacter tropicalis]
VSAYLLDTNIISDLVRNPSGPCARRIERIDPGDLCTSIIVAAELRYGCAKKGSAKLLNRVESVLVFIPVLPLDIPADCEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMRIRGLKVENWLEDGT

Nucleotide

• Length: 411 bp

>T79608 NZ_CP022700:68376-68786 [Acetobacter tropicalis]
GTGAGCGCCTACCTCCTTGATACCAATATCATCAGTGATCTGGTTCGCAATCCTTCCGGCCCGTGCGCGCGTCGCATCGA
ACGTATCGATCCCGGCGACCTGTGTACCAGCATCATTGTGGCCGCTGAGTTACGATATGGATGTGCTAAAAAAGGCTCTG
CAAAGCTGTTAAACCGGGTTGAAAGCGTGCTGGTGTTTATCCCCGTGCTGCCCCTCGATATTCCTGCCGACTGTGAATAC
GGTGGTATCCGCGCCGAACTCGAAGCCGCAGGGCAGACGATCGGCATGAATGATCTGCTCATCGCGGCTCACGCCTACAC
CCTCAAGGCGACCCTGGTGACGAACAACACACGTGAGTTTATGCGCATCCGAGGCTTGAAGGTTGAAAACTGGCTGGAGG
ATGGGACGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10374.92 Da
• Isoelectric Point: 4.4463

>AT79608 WP_176373091.1 NZ_CP022700:68107-68379 [Acetobacter tropicalis]
MPVPEVTPRPREVKLFRNNRSQAVRIPVEFEMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLSPEDDFPDIQ
DVAATPEDIL

Nucleotide

• Length: 273 bp

>AT79608 NZ_CP022700:68107-68379 [Acetobacter tropicalis]
ATGCCTGTTCCTGAAGTGACTCCCCGACCGCGCGAAGTGAAACTCTTTCGGAACAATCGCAGTCAGGCTGTGCGCATTCC
GGTCGAATTTGAAATGCCAGGAGATCGCGTGCTGATCCGCCGTGACGGTGACAAGCTCGTTCTGGAGCCTGTGAAAACCC
CTTCAACGTTGAAGGAGCTTCTCATGGCATGGCGCGAAGAGCCGCAGCTCAGTCCGGAAGATGATTTTCCTGATATTCAG
GACGTTGCCGCCACACCGGAAGATATTCTGTGA