Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2985694..2986348 Replicon chromosome
Accession NZ_CP022689
Organism Escherichia coli strain CDC#03-98

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RM34_RS15965 Protein ID WP_000244781.1
Coordinates 2985941..2986348 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RM34_RS15960 Protein ID WP_000354046.1
Coordinates 2985694..2985960 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RM34_RS15940 2981782..2983215 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
RM34_RS15945 2983260..2983571 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
RM34_RS15950 2983735..2984394 + 660 WP_000250274.1 hemolysin III family protein -
RM34_RS15955 2984471..2985451 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
RM34_RS15960 2985694..2985960 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RM34_RS15965 2985941..2986348 + 408 WP_000244781.1 protein YgfX Toxin
RM34_RS15970 2986388..2986909 - 522 WP_001055874.1 flavodoxin FldB -
RM34_RS15975 2987021..2987917 + 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
RM34_RS15980 2987942..2988652 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RM34_RS15985 2988658..2990391 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T79569 WP_000244781.1 NZ_CP022689:2985941-2986348 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T79569 NZ_CP022689:2985941-2986348 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT79569 WP_000354046.1 NZ_CP022689:2985694-2985960 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT79569 NZ_CP022689:2985694-2985960 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References