Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4684627..4685428 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0H2V687
Locus tag CIW80_RS23495 Protein ID WP_000854739.1
Coordinates 4685048..4685428 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag CIW80_RS23490 Protein ID WP_001285482.1
Coordinates 4684627..4685001 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS23455 4680715..4681170 + 456 WP_000581502.1 hypothetical protein -
CIW80_RS26140 4681289..4681500 - 212 Protein_4286 hypothetical protein -
CIW80_RS23465 4681581..4682402 + 822 WP_001234710.1 DUF945 domain-containing protein -
CIW80_RS23470 4682665..4683138 + 474 WP_000855076.1 antirestriction protein -
CIW80_RS23475 4683154..4683630 + 477 WP_001186170.1 RadC family protein -
CIW80_RS23480 4683693..4683914 + 222 WP_000692312.1 DUF987 domain-containing protein -
CIW80_RS23485 4683933..4684577 + 645 WP_000094915.1 hypothetical protein -
CIW80_RS23490 4684627..4685001 + 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CIW80_RS23495 4685048..4685428 + 381 WP_000854739.1 TA system toxin CbtA family protein Toxin
CIW80_RS23500 4685425..4685913 + 489 WP_001054232.1 hypothetical protein -
CIW80_RS23505 4685933..4686130 + 198 WP_000772027.1 DUF957 domain-containing protein -
CIW80_RS23510 4686215..4687042 + 828 WP_001304477.1 DUF4942 domain-containing protein -
CIW80_RS23520 4687529..4688467 + 939 WP_000351287.1 glyoxylate/hydroxypyruvate reductase GhrA -
CIW80_RS23525 4688522..4689259 + 738 WP_000283664.1 zinc-binding phosphatase -
CIW80_RS23530 4689283..4689837 + 555 WP_001001909.1 molecular chaperone YcdY -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 4659859..4695370 35511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 14214.14 Da        Isoelectric Point: 6.4773

>T79550 WP_000854739.1 NZ_CP022686:4685048-4685428 [Escherichia coli Nissle 1917]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Download         Length: 381 bp

>T79550 NZ_CP022686:4685048-4685428 [Escherichia coli Nissle 1917]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT79550 WP_001285482.1 NZ_CP022686:4684627-4685001 [Escherichia coli Nissle 1917]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT79550 NZ_CP022686:4684627-4685001 [Escherichia coli Nissle 1917]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H2V687


Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References