Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3746623..3747421 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag CIW80_RS18725 Protein ID WP_000854730.1
Coordinates 3746623..3747000 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag CIW80_RS18730 Protein ID WP_001285481.1
Coordinates 3747047..3747421 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS18695 3741767..3743171 + 1405 Protein_3432 IS66 family transposase -
CIW80_RS18700 3743425..3744558 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
CIW80_RS18705 3744619..3744747 + 129 Protein_3434 transposase domain-containing protein -
CIW80_RS18710 3744731..3744919 + 189 Protein_3435 IS66 family transposase -
CIW80_RS18715 3745067..3746055 + 989 Protein_3436 IS630 family transposase -
CIW80_RS18720 3746134..3746626 - 493 Protein_3437 hypothetical protein -
CIW80_RS18725 3746623..3747000 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
CIW80_RS18730 3747047..3747421 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CIW80_RS18735 3747471..3748115 - 645 WP_000086759.1 hypothetical protein -
CIW80_RS18740 3748134..3748355 - 222 WP_000692315.1 DUF987 domain-containing protein -
CIW80_RS18745 3748418..3748894 - 477 WP_001313574.1 RadC family protein -
CIW80_RS18750 3748910..3749383 - 474 WP_001313575.1 antirestriction protein -
CIW80_RS18755 3749477..3749722 - 246 WP_001164966.1 hypothetical protein -
CIW80_RS18760 3749722..3750543 - 822 WP_024199417.1 DUF945 domain-containing protein -
CIW80_RS18765 3750643..3750876 - 234 WP_001213776.1 DUF905 family protein -
CIW80_RS18770 3750994..3751446 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T79548 WP_000854730.1 NZ_CP022686:c3747000-3746623 [Escherichia coli Nissle 1917]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T79548 NZ_CP022686:c3747000-3746623 [Escherichia coli Nissle 1917]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT79548 WP_001285481.1 NZ_CP022686:c3747421-3747047 [Escherichia coli Nissle 1917]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT79548 NZ_CP022686:c3747421-3747047 [Escherichia coli Nissle 1917]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References