Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3547625..3548175 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag CIW80_RS17770 Protein ID WP_001160966.1
Coordinates 3547861..3548175 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CIW80_RS17765 Protein ID WP_000125566.1
Coordinates 3547625..3547858 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS17740 3542698..3542985 + 288 WP_000203741.1 ferredoxin-like protein FixX -
CIW80_RS17745 3543044..3544375 + 1332 WP_001183207.1 MFS transporter -
CIW80_RS17750 3544483..3545013 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CIW80_RS17755 3545006..3546868 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
CIW80_RS17760 3547060..3547539 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
CIW80_RS17765 3547625..3547858 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CIW80_RS17770 3547861..3548175 + 315 WP_001160966.1 CcdB family protein Toxin
CIW80_RS17775 3548172..3549020 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CIW80_RS17780 3549027..3549404 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CIW80_RS17785 3549407..3550228 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CIW80_RS17790 3550225..3551214 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CIW80_RS17795 3551214..3552500 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T79547 WP_001160966.1 NZ_CP022686:3547861-3548175 [Escherichia coli Nissle 1917]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T79547 NZ_CP022686:3547861-3548175 [Escherichia coli Nissle 1917]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT79547 WP_000125566.1 NZ_CP022686:3547625-3547858 [Escherichia coli Nissle 1917]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT79547 NZ_CP022686:3547625-3547858 [Escherichia coli Nissle 1917]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References