Detailed information of TA system TA079543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3392627..3393447
• Replicon: chromosome
• Accession: NZ_CP022686
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: CIW80_RS16965
• Protein ID: WP_001054379.1
• Coordinates: 3393190..3393447 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: CIW80_RS16960
• Protein ID: WP_000123957.1
• Coordinates: 3392627..3393178 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS16935	3388321..3389634	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
CIW80_RS16940	3389646..3389924	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CIW80_RS16945	3389921..3391042	-	1122	WP_000010833.1	M42 family metallopeptidase	-
CIW80_RS26065	3391287..3391403	-	117	Protein_3106	VOC family protein	-
CIW80_RS16950	3391441..3391701	-	261	WP_000077645.1	hypothetical protein	-
CIW80_RS16955	3391829..3392575	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
CIW80_RS16960	3392627..3393178	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
CIW80_RS16965	3393190..3393447	-	258	WP_001054379.1	YjhX family toxin	Toxin
CIW80_RS16975	3393824..3393988	-	165	Protein_3111	GNAT family N-acetyltransferase	-
CIW80_RS16985	3395294..3396337	+	1044	WP_001363826.1	hypothetical protein	-
CIW80_RS16990	3396920..3397900	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T79543 WP_001054379.1 NZ_CP022686:c3393447-3393190 [Escherichia coli Nissle 1917]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T79543 NZ_CP022686:c3393447-3393190 [Escherichia coli Nissle 1917]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT79543 WP_000123957.1 NZ_CP022686:c3393178-3392627 [Escherichia coli Nissle 1917]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT79543 NZ_CP022686:c3393178-3392627 [Escherichia coli Nissle 1917]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030