Detailed information of TA system TA079542

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3359990..3360825
• Replicon: chromosome
• Accession: NZ_CP022686
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: CIW80_RS16795
• Protein ID: WP_000854759.1
• Coordinates: 3360448..3360825 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CIW80_RS16790
• Protein ID: WP_001295723.1
• Coordinates: 3359990..3360358 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS16755	3357105..3357263	+	159	WP_001323397.1	DUF905 family protein	-
CIW80_RS16765	3357418..3358236	+	819	WP_001234738.1	DUF945 domain-containing protein	-
CIW80_RS16775	3358578..3359051	+	474	WP_001350782.1	antirestriction protein	-
CIW80_RS16780	3359067..3359543	+	477	WP_001186775.1	RadC family protein	-
CIW80_RS16785	3359606..3359827	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CIW80_RS16790	3359990..3360358	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CIW80_RS16795	3360448..3360825	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
CIW80_RS16800	3360822..3361310	+	489	WP_000761690.1	hypothetical protein	-
CIW80_RS16805	3361327..3361503	+	177	WP_000839286.1	DUF957 domain-containing protein	-
CIW80_RS26060	3361603..3361761	+	159	WP_001467148.1	hypothetical protein	-
CIW80_RS16815	3362125..3362301	+	177	Protein_3081	helix-turn-helix domain-containing protein	-
CIW80_RS16820	3363074..3364714	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3346532..3373724	27192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T79542 WP_000854759.1 NZ_CP022686:3360448-3360825 [Escherichia coli Nissle 1917]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T79542 NZ_CP022686:3360448-3360825 [Escherichia coli Nissle 1917]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT79542 WP_001295723.1 NZ_CP022686:3359990-3360358 [Escherichia coli Nissle 1917]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT79542 NZ_CP022686:3359990-3360358 [Escherichia coli Nissle 1917]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52