Detailed information of TA system TA079539

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2597235..2598026
• Replicon: chromosome
• Accession: NZ_CP022686
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: CIW80_RS13010
• Protein ID: WP_000691791.1
• Coordinates: 2597601..2598026 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: CIW80_RS13005
• Protein ID: WP_000939437.1
• Coordinates: 2597235..2597570 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS12980	2593186..2593404	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
CIW80_RS12985	2593522..2594136	-	615	WP_000772910.1	inovirus Gp2 family protein	-
CIW80_RS12990	2594729..2595682	+	954	WP_000290406.1	hypothetical protein	-
CIW80_RS12995	2595918..2596732	+	815	Protein_2395	DUF945 domain-containing protein	-
CIW80_RS13000	2596762..2597235	+	474	WP_001292742.1	DNA repair protein RadC	-
CIW80_RS13005	2597235..2597570	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CIW80_RS13010	2597601..2598026	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
CIW80_RS13015	2598047..2599238	+	1192	Protein_2399	AAA family ATPase	-
CIW80_RS13020	2599223..2599444	+	222	WP_125112077.1	hypothetical protein	-
CIW80_RS13025	2599441..2600172	+	732	WP_001216639.1	TIGR02646 family protein	-
CIW80_RS13035	2600795..2601181	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
CIW80_RS13045	2601863..2602360	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2576577..2602360	25783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T79539 WP_000691791.1 NZ_CP022686:2597601-2598026 [Escherichia coli Nissle 1917]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T79539 NZ_CP022686:2597601-2598026 [Escherichia coli Nissle 1917]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT79539 WP_000939437.1 NZ_CP022686:2597235-2597570 [Escherichia coli Nissle 1917]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT79539 NZ_CP022686:2597235-2597570 [Escherichia coli Nissle 1917]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6