Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1660780..1661434 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CIW80_RS08155 Protein ID WP_000244781.1
Coordinates 1660780..1661187 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CIW80_RS08160 Protein ID WP_000354046.1
Coordinates 1661168..1661434 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS08135 1656737..1658470 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
CIW80_RS08140 1658476..1659186 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CIW80_RS08145 1659211..1660107 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CIW80_RS08150 1660219..1660740 + 522 WP_001055874.1 flavodoxin FldB -
CIW80_RS08155 1660780..1661187 - 408 WP_000244781.1 protein YgfX Toxin
CIW80_RS08160 1661168..1661434 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CIW80_RS08165 1661677..1662657 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
CIW80_RS08170 1662734..1663393 - 660 WP_000250274.1 hemolysin III family protein -
CIW80_RS08175 1663557..1663868 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CIW80_RS08180 1663913..1665346 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T79536 WP_000244781.1 NZ_CP022686:c1661187-1660780 [Escherichia coli Nissle 1917]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T79536 NZ_CP022686:c1661187-1660780 [Escherichia coli Nissle 1917]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT79536 WP_000354046.1 NZ_CP022686:c1661434-1661168 [Escherichia coli Nissle 1917]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT79536 NZ_CP022686:c1661434-1661168 [Escherichia coli Nissle 1917]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References