Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3971826..3972480 Replicon chromosome
Accession NZ_CP022672
Organism Shigella sonnei strain 866

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CH310_RS20185 Protein ID WP_000244777.1
Coordinates 3971826..3972233 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CH310_RS20190 Protein ID WP_000354046.1
Coordinates 3972214..3972480 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH310_RS20165 (3967783) 3967783..3969516 - 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -
CH310_RS20170 (3969522) 3969522..3970232 - 711 WP_052983156.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CH310_RS20175 (3970257) 3970257..3971153 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CH310_RS20180 (3971265) 3971265..3971786 + 522 WP_001055874.1 flavodoxin FldB -
CH310_RS20185 (3971826) 3971826..3972233 - 408 WP_000244777.1 protein YgfX Toxin
CH310_RS20190 (3972214) 3972214..3972480 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CH310_RS20195 (3972723) 3972723..3973703 + 981 WP_000886060.1 tRNA-modifying protein YgfZ -
CH310_RS20200 (3973899) 3973899..3974558 - 660 WP_000250274.1 hemolysin III family protein -
CH310_RS20205 (3974722) 3974722..3975033 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CH310_RS20210 (3975078) 3975078..3976511 + 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
CH310_RS20215 (3976568) 3976568..3977311 - 744 WP_000951938.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T79502 WP_000244777.1 NZ_CP022672:c3972233-3971826 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T79502 NZ_CP022672:c3972233-3971826 [Shigella sonnei]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT79502 WP_000354046.1 NZ_CP022672:c3972480-3972214 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT79502 NZ_CP022672:c3972480-3972214 [Shigella sonnei]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References