Detailed information of TA system TA079488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1168861..1169698
• Replicon: chromosome
• Accession: NZ_CP022672
• Organism: Shigella sonnei strain 866

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CH310_RS05695
• Protein ID: WP_000227784.1
• Coordinates: 1168861..1169403 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CH310_RS05700
• Protein ID: WP_001297137.1
• Coordinates: 1169387..1169698 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH310_RS05665 (1163925)	1163925..1164416	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
CH310_RS05670 (1164544)	1164544..1165908	-	1365	WP_001000971.1	MFS transporter	-
CH310_RS05675 (1166027)	1166027..1166724	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
CH310_RS05680 (1167093)	1167093..1167491	+	399	Protein_1095	SEL1-like repeat protein	-
CH310_RS05690 (1168239)	1168239..1168805	+	567	Protein_1097	SEL1-like repeat protein	-
CH310_RS05695 (1168861)	1168861..1169403	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CH310_RS05700 (1169387)	1169387..1169698	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CH310_RS05705 (1169883)	1169883..1170773	-	891	WP_000971327.1	heme o synthase	-
CH310_RS05710 (1170785)	1170785..1171114	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CH310_RS05715 (1171114)	1171114..1171728	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CH310_RS05720 (1171718)	1171718..1173709	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CH310_RS05725 (1173731)	1173731..1174678	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T79488 WP_000227784.1 NZ_CP022672:c1169403-1168861 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T79488 NZ_CP022672:c1169403-1168861 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT79488 WP_001297137.1 NZ_CP022672:c1169698-1169387 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT79488 NZ_CP022672:c1169698-1169387 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3