Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 472600..473097 Replicon chromosome
Accession NZ_CP022672
Organism Shigella sonnei strain 866

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CH310_RS02275 Protein ID WP_001173343.1
Coordinates 472924..473097 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CH310_RS02270 Protein ID WP_000398619.1
Coordinates 472600..472896 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH310_RS02250 (469331) 469331..470050 - 720 WP_000611285.1 two-component system response regulator DcuR -
CH310_RS02255 (470047) 470047..471678 - 1632 WP_001216495.1 sensor histidine kinase -
CH310_RS02260 (471859) 471859..472089 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
CH310_RS02265 (472101) 472101..472373 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
CH310_RS02270 (472600) 472600..472896 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CH310_RS02275 (472924) 472924..473097 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CH310_RS02280 (473216) 473216..474733 - 1518 WP_001295086.1 lysine--tRNA ligase -
CH310_RS02285 (474970) 474970..476427 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
CH310_RS02290 (476486) 476486..476743 - 258 Protein_429 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T79486 WP_001173343.1 NZ_CP022672:472924-473097 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T79486 NZ_CP022672:472924-473097 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT79486 WP_000398619.1 NZ_CP022672:472600-472896 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT79486 NZ_CP022672:472600-472896 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References