Detailed information of TA system TA079485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 86941..87596
• Replicon: plasmid pPR4
• Accession: NZ_CP022669
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1217

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CHR56_RS36950
• Protein ID: WP_077988169.1
• Coordinates: 87177..87596 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7W6UNS6
• Locus tag: CHR56_RS36945
• Protein ID: WP_018496487.1
• Coordinates: 86941..87177 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR56_RS36915	82084..82752	+	669	WP_131624552.1	hypothetical protein	-
CHR56_RS36920	82862..83140	+	279	WP_018068327.1	hypothetical protein	-
CHR56_RS39775	83385..83789	+	405	WP_131624554.1	hypothetical protein	-
CHR56_RS39780	83967..84440	+	474	WP_131624556.1	hypothetical protein	-
CHR56_RS36935	84578..85579	+	1002	WP_077988171.1	hypothetical protein	-
CHR56_RS36940	85664..86680	+	1017	WP_131645741.1	hypothetical protein	-
CHR56_RS36945	86941..87177	+	237	WP_018496487.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CHR56_RS36950	87177..87596	+	420	WP_077988169.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHR56_RS36955	87852..88100	+	249	Protein_88	amidase	-
CHR56_RS36960	88232..89830	-	1599	WP_077988167.1	asparagine synthase	-
CHR56_RS40370	89903..90061	-	159	WP_165505241.1	hypothetical protein	-
CHR56_RS36965	90410..90964	-	555	WP_077988166.1	GNAT family N-acetyltransferase	-
CHR56_RS36970	91541..92236	+	696	WP_077988165.1	protein rhiA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..327451	327451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15660.87 Da
• Isoelectric Point: 4.7852

>T79485 WP_077988169.1 NZ_CP022669:87177-87596 [Rhizobium leguminosarum bv. viciae]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPERELDNLSALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T79485 NZ_CP022669:87177-87596 [Rhizobium leguminosarum bv. viciae]
ATGGCGCGCTACATGCTCGATACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTACGGTGTTTCCGCATCCGATGATC
CGGAACGAGAACTTGATAATCTCTCCGCCCTGGCCGAGCTAATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGAAAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGTATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9051.15 Da
• Isoelectric Point: 5.6454

>AT79485 WP_018496487.1 NZ_CP022669:86941-87177 [Rhizobium leguminosarum bv. viciae]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQDERDKI

Nucleotide

• Length: 237 bp

>AT79485 NZ_CP022669:86941-87177 [Rhizobium leguminosarum bv. viciae]
ATGCACACCACTAAAGCTTTCAAGAATGGAAATTCCCAAGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAGATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGTGACGGAAGAGAGTCCCATGAGCAAGACGAGCGGGACAAGATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6UNS6