Detailed information of TA system TA079484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 16770..18246
• Replicon: plasmid pPR4
• Accession: NZ_CP022669
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1217

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: CHR56_RS36610
• Protein ID: WP_094231109.1
• Coordinates: 17380..18246 (+)
• Length: 289 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: CHR56_RS36605
• Protein ID: WP_077988207.1
• Coordinates: 16770..17387 (+)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR56_RS36575	12144..12956	+	813	WP_077988212.1	P-type conjugative transfer protein TrbG	-
CHR56_RS36580	12960..13400	+	441	WP_077988211.1	conjugal transfer protein TrbH	-
CHR56_RS36585	13412..14710	+	1299	WP_077988210.1	IncP-type conjugal transfer protein TrbI	-
CHR56_RS36590	15021..15725	+	705	WP_077988209.1	autoinducer binding domain-containing protein	-
CHR56_RS36595	15729..16019	-	291	WP_077988208.1	transcriptional repressor TraM	-
CHR56_RS36600	16279..16527	+	249	WP_027668121.1	helix-turn-helix transcriptional regulator	-
CHR56_RS36605	16770..17387	+	618	WP_077988207.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CHR56_RS36610	17380..18246	+	867	WP_094231109.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CHR56_RS36615	18377..18994	-	618	WP_077988205.1	conjugal transfer protein TraH	-
CHR56_RS36620	19011..20174	-	1164	WP_077988204.1	conjugal transfer protein TraB	-
CHR56_RS36625	20164..20730	-	567	WP_026236762.1	conjugative transfer signal peptidase TraF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..327451	327451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-263)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (15-56)

Sequences

Toxin

Protein

• Length: 289 a.a.
• Molecular weight: 32142.89 Da
• Isoelectric Point: 5.4178

>T79484 WP_094231109.1 NZ_CP022669:17380-18246 [Rhizobium leguminosarum bv. viciae]
MAKEIRNVGASVRARLLQLAKASGQSFDLVLTRFALERLLFRLSQSPHAGRFVLKGAMLMMGWFDNPHRGTRDLDLLGFG
DPSPGPMLETFREILAQEADDGVTFDADTLHVDRIREALDYGGLRLQVIASISGARINLTIDIGFGDALEPGVEVLDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFSDDRLARAIAATFDRRATSIPIDLPDALTD
AFAKDQQKQRQWRAFVEGVAHNPGDLTDVIAEIATFLMPHAVAAARLD

Nucleotide

• Length: 867 bp

>T79484 NZ_CP022669:17380-18246 [Rhizobium leguminosarum bv. viciae]
ATGGCTAAAGAAATCAGAAACGTCGGTGCTTCGGTTCGCGCCCGCCTGTTGCAACTCGCCAAGGCAAGCGGACAAAGCTT
CGATCTGGTCCTGACGCGTTTCGCTCTTGAACGGTTATTGTTCCGGCTTAGCCAGTCGCCCCACGCCGGTCGCTTCGTTC
TAAAAGGGGCGATGTTGATGATGGGTTGGTTCGACAATCCGCACCGAGGCACCCGCGACCTCGATCTTTTGGGTTTCGGT
GATCCGAGTCCGGGCCCGATGCTTGAGACATTTCGGGAAATTCTGGCGCAAGAGGCCGACGATGGCGTTACGTTTGACGC
TGATACGCTGCATGTGGATCGTATTCGCGAGGCATTGGATTATGGTGGGCTCAGGCTGCAGGTGATCGCTTCGATCAGCG
GAGCCCGGATCAACCTGACGATCGACATCGGTTTTGGCGATGCGCTTGAGCCTGGGGTGGAGGTTTTGGATTATCCTTCT
ATGCTCGATTTTCCGATGCCGCGGCTTCGGGCCTATGCGCGAGAAACGGTCATTGCCGAGAAGTTCCAGGCAATGGTGAT
GCTGGGGCGAGTAAACAGCCGCATGAAGGATTTCTACGACATCTGGATCCTCAGCAGGTCTTTCGACTTCAGCGATGATC
GGCTGGCGCGCGCAATAGCCGCTACCTTTGATCGCCGCGCGACATCGATCCCCATTGACCTCCCCGACGCCCTAACGGAC
GCCTTTGCCAAGGACCAGCAAAAGCAGCGGCAATGGCGAGCTTTCGTTGAAGGCGTTGCACACAATCCTGGCGATCTGAC
AGACGTCATCGCGGAGATTGCCACATTCCTGATGCCGCACGCGGTCGCGGCTGCGAGGCTGGACTAG

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22439.99 Da
• Isoelectric Point: 10.4301

>AT79484 WP_077988207.1 NZ_CP022669:16770-17387 [Rhizobium leguminosarum bv. viciae]
MPRPVTQRQIARTVLTERGIARLVELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAQLDVNHSLAEVAKRAPKGVICL
VSALAFHGLTDQLPGQIWLAIARKDWAPKLDSPAMRIVRFTDSLLNESVETHVIEGVPVRVFGIAKTIADCFRYRNKIGL
SVAIEGLQEALRQRRATPGEIANQAERGGAGSVIRPYLEALTANG

Nucleotide

• Length: 618 bp

>AT79484 NZ_CP022669:16770-17387 [Rhizobium leguminosarum bv. viciae]
ATGCCCAGGCCCGTCACGCAACGCCAAATTGCCCGCACCGTGCTAACTGAGCGCGGAATAGCACGTTTGGTGGAACTGCG
GAACGCAGGTGTGACGGCTGCCACCATGAGCCGAATGGAACGAGATGGTGAAGTGCTCCGGCTTGCCCGCGGTCTTTATC
AACTTCCTGATGCGCAGCTCGACGTCAACCACAGTCTGGCGGAGGTTGCCAAACGCGCGCCAAAAGGCGTTATCTGCCTC
GTTTCAGCATTGGCGTTCCACGGCCTGACAGATCAGCTCCCGGGACAAATCTGGCTTGCCATAGCCCGTAAGGACTGGGC
TCCGAAACTGGACAGCCCCGCTATGCGCATCGTGCGTTTCACAGACAGCCTTCTCAACGAAAGCGTCGAAACCCATGTCA
TTGAAGGCGTTCCTGTGAGGGTGTTTGGAATTGCCAAGACAATTGCCGATTGCTTTCGCTATCGCAACAAGATCGGCCTT
TCAGTAGCGATAGAAGGGCTGCAGGAGGCGCTGCGGCAACGAAGGGCTACTCCTGGCGAAATCGCCAACCAGGCCGAACG
CGGTGGCGCCGGATCGGTGATCCGGCCGTATCTCGAGGCGCTGACCGCCAATGGCTAA