Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 515838..516610 | Replicon | plasmid pPR2 |
| Accession | NZ_CP022667 | ||
| Organism | Rhizobium leguminosarum bv. viciae strain BIHB 1217 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A2Z4YVU8 |
| Locus tag | CHR56_RS33390 | Protein ID | WP_018246328.1 |
| Coordinates | 515838..516326 (-) | Length | 163 a.a. |
Antitoxin (Protein)
| Gene name | TacA2 | Uniprot ID | A0A7Y3WKI8 |
| Locus tag | CHR56_RS33395 | Protein ID | WP_026188748.1 |
| Coordinates | 516326..516610 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CHR56_RS33345 | 510961..511590 | + | 630 | WP_026188749.1 | J domain-containing protein | - |
| CHR56_RS33350 | 511610..512311 | - | 702 | WP_018246336.1 | DUF899 family protein | - |
| CHR56_RS39730 | 512304..512522 | - | 219 | WP_128409058.1 | hypothetical protein | - |
| CHR56_RS40315 | 513196..513522 | - | 327 | WP_155256937.1 | hypothetical protein | - |
| CHR56_RS33370 | 513816..514079 | - | 264 | WP_018246331.1 | DUF982 domain-containing protein | - |
| CHR56_RS33380 | 514733..514993 | + | 261 | WP_018246330.1 | DUF982 domain-containing protein | - |
| CHR56_RS33385 | 515024..515548 | - | 525 | WP_018517806.1 | hypothetical protein | - |
| CHR56_RS33390 | 515838..516326 | - | 489 | WP_018246328.1 | GNAT family N-acetyltransferase | Toxin |
| CHR56_RS33395 | 516326..516610 | - | 285 | WP_026188748.1 | DUF1778 domain-containing protein | Antitoxin |
| CHR56_RS33405 | 517094..517843 | - | 750 | WP_018246326.1 | sulfite exporter TauE/SafE family protein | - |
| CHR56_RS33410 | 517951..519018 | - | 1068 | WP_018246325.1 | iron ABC transporter permease | - |
| CHR56_RS33415 | 519008..521416 | - | 2409 | WP_018246324.1 | ABC transporter permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..605219 | 605219 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 163 a.a. Molecular weight: 17293.88 Da Isoelectric Point: 7.9271
>T79483 WP_018246328.1 NZ_CP022667:c516326-515838 [Rhizobium leguminosarum bv. viciae]
MLSAPTLLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGERVVGYYCLSSGALAVSDAPGAIRRNMPDPV
PMAVLGRLAIDRNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHFGFAASPANPMALVLSLKAQVG
LK
MLSAPTLLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGERVVGYYCLSSGALAVSDAPGAIRRNMPDPV
PMAVLGRLAIDRNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHFGFAASPANPMALVLSLKAQVG
LK
Download Length: 489 bp
>T79483 NZ_CP022667:c516326-515838 [Rhizobium leguminosarum bv. viciae]
ATGTTGTCTGCACCGACGCTTCTCGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTGCGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTCTGCGAGGGCGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTGGCGGTCTCGGACGCTCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCCATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCGATCTCCGATGAGGCCAAGG
CCTTCTATGAGCATTTTGGATTTGCAGCGTCGCCCGCGAATCCTATGGCCTTGGTGTTATCATTGAAAGCTCAAGTAGGC
CTGAAGTGA
ATGTTGTCTGCACCGACGCTTCTCGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTGCGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTCTGCGAGGGCGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTGGCGGTCTCGGACGCTCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCCATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCGATCTCCGATGAGGCCAAGG
CCTTCTATGAGCATTTTGGATTTGCAGCGTCGCCCGCGAATCCTATGGCCTTGGTGTTATCATTGAAAGCTCAAGTAGGC
CTGAAGTGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10687.05 Da Isoelectric Point: 8.6086
>AT79483 WP_026188748.1 NZ_CP022667:c516610-516326 [Rhizobium leguminosarum bv. viciae]
MTSKNATDTHTRAVNLRVREDVRVLIDRAAKAHGKTRSDFMIDASRRAAEDALLDQTLVRVDPDSYKHYLAVLDQPPNSE
GFERLMNAPKPWQP
MTSKNATDTHTRAVNLRVREDVRVLIDRAAKAHGKTRSDFMIDASRRAAEDALLDQTLVRVDPDSYKHYLAVLDQPPNSE
GFERLMNAPKPWQP
Download Length: 285 bp
>AT79483 NZ_CP022667:c516610-516326 [Rhizobium leguminosarum bv. viciae]
ATGACCAGCAAAAATGCCACTGATACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGCGCCGCCAAGGCCCACGGGAAAACCCGTTCGGATTTCATGATCGACGCATCGCGCCGTGCTGCAGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAGCCGCCAAATAGCGAA
GGCTTCGAACGGTTGATGAATGCTCCCAAACCCTGGCAGCCGTAA
ATGACCAGCAAAAATGCCACTGATACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGCGCCGCCAAGGCCCACGGGAAAACCCGTTCGGATTTCATGATCGACGCATCGCGCCGTGCTGCAGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAGCCGCCAAATAGCGAA
GGCTTCGAACGGTTGATGAATGCTCCCAAACCCTGGCAGCCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z4YVU8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Y3WKI8 |