Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 762021..762712 Replicon plasmid pPR1
Accession NZ_CP022666
Organism Rhizobium leguminosarum bv. viciae strain BIHB 1217

Toxin (Protein)


Gene name vapC Uniprot ID A0A6P0D4X9
Locus tag CHR56_RS29085 Protein ID WP_018245712.1
Coordinates 762021..762449 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R0AD13
Locus tag CHR56_RS29090 Protein ID WP_017991284.1
Coordinates 762446..762712 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR56_RS29055 757058..757861 - 804 WP_018245717.1 crotonase/enoyl-CoA hydratase family protein -
CHR56_RS29060 757950..758534 + 585 WP_026188704.1 TetR/AcrR family transcriptional regulator -
CHR56_RS29065 758807..759646 + 840 WP_018245715.1 transporter substrate-binding domain-containing protein -
CHR56_RS29070 759814..760476 + 663 WP_026188703.1 amino acid ABC transporter permease -
CHR56_RS29075 760483..761142 + 660 WP_011648868.1 amino acid ABC transporter permease -
CHR56_RS29080 761155..761925 + 771 WP_018245713.1 amino acid ABC transporter ATP-binding protein -
CHR56_RS29085 762021..762449 - 429 WP_018245712.1 type II toxin-antitoxin system VapC family toxin Toxin
CHR56_RS29090 762446..762712 - 267 WP_017991284.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CHR56_RS29095 762969..763718 + 750 WP_018245711.1 GntR family transcriptional regulator -
CHR56_RS29100 763722..764507 + 786 WP_018245710.1 transporter substrate-binding domain-containing protein -
CHR56_RS29105 764588..765259 + 672 WP_094231084.1 amino acid ABC transporter permease -
CHR56_RS29110 765256..765915 + 660 WP_018245708.1 amino acid ABC transporter permease -
CHR56_RS29115 765893..766618 + 726 WP_018245707.1 amino acid ABC transporter ATP-binding protein -
CHR56_RS29120 766767..767138 - 372 WP_018245706.1 type II toxin-antitoxin system RelE/ParE family toxin -
CHR56_RS29125 767138..767395 - 258 WP_018245705.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1169137 1169137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15914.18 Da        Isoelectric Point: 4.6772

>T79480 WP_018245712.1 NZ_CP022666:c762449-762021 [Rhizobium leguminosarum bv. viciae]
VRLLLDTNVLSEVTRPRPDAHVLQWLDSLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGFEIELVDPWTERP

Download         Length: 429 bp

>T79480 NZ_CP022666:c762449-762021 [Rhizobium leguminosarum bv. viciae]
GTGAGACTTCTGCTGGACACTAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACATGTCCTACAATGGCTGGA
CAGTCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GCCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAGCGCTTCGAGCAGCGTGTCATCCCTGTGGAT
CAGCCGGTTGCGATAGCTTGGGGCGACTTGATGGGGCTCGCAAAGCGAAGCGGGCGCGGCTTGTCGTCAATGGATGGCCT
GATTGCCGCCACCGCGATTGCTCACGATCTCACCCTCGCCACTCGCAATATAAAGGATTTCGAGGGCTTTGAGATCGAGC
TTGTCGACCCTTGGACCGAGCGCCCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9960.34 Da        Isoelectric Point: 10.0842

>AT79480 WP_017991284.1 NZ_CP022666:c762712-762446 [Rhizobium leguminosarum bv. viciae]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT79480 NZ_CP022666:c762712-762446 [Rhizobium leguminosarum bv. viciae]
ATGCAGCCATCACGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACGCCGCAAACAATCATGCGCAATGGCAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCTGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTGATCTTCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P0D4X9

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0AD13

References