Detailed information of TA system TA079479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 178795..179375
• Replicon: plasmid pPR1
• Accession: NZ_CP022666
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1217

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A444I7Z5
• Locus tag: CHR56_RS26345
• Protein ID: WP_018245324.1
• Coordinates: 178795..179178 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y3WNA0
• Locus tag: CHR56_RS26350
• Protein ID: WP_018245323.1
• Coordinates: 179175..179375 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR56_RS26310	174243..174463	-	221	Protein_169	Gfo/Idh/MocA family oxidoreductase	-
CHR56_RS26320	174879..175973	+	1095	WP_094231074.1	hypothetical protein	-
CHR56_RS26325	176270..177322	-	1053	WP_018245326.1	ribosome small subunit-dependent GTPase A	-
CHR56_RS40205	177357..177533	-	177	WP_018071841.1	hypothetical protein	-
CHR56_RS40210	177710..178039	+	330	WP_018517229.1	hypothetical protein	-
CHR56_RS26335	178358..178585	-	228	WP_085990123.1	CopG family transcriptional regulator	-
CHR56_RS26345	178795..179178	-	384	WP_018245324.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHR56_RS26350	179175..179375	-	201	WP_018245323.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CHR56_RS40215	179540..179779	-	240	WP_155256963.1	hypothetical protein	-
CHR56_RS26360	179912..180229	+	318	Protein_178	glycerol-3-phosphate dehydrogenase	-
CHR56_RS26365	180378..180509	-	132	Protein_179	transposase domain-containing protein	-
CHR56_RS26370	180569..181201	+	633	WP_018245319.1	DUF433 domain-containing protein	-
CHR56_RS26375	181198..181572	+	375	WP_018245318.1	DUF5615 family PIN-like protein	-
CHR56_RS26380	181644..183224	-	1581	WP_018245317.1	ABC transporter substrate-binding protein	-
CHR56_RS26385	183346..184131	+	786	WP_018245316.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1169137	1169137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14117.31 Da
• Isoelectric Point: 9.1808

>T79479 WP_018245324.1 NZ_CP022666:c179178-178795 [Rhizobium leguminosarum bv. viciae]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Nucleotide

• Length: 384 bp

>T79479 NZ_CP022666:c179178-178795 [Rhizobium leguminosarum bv. viciae]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATGTTGATGCGGAACTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTAGTTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCAGCCCAGCGCCAAGCGTTCGTCGCAACGCATGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATACTGCTTGATCGGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTTCAGGCGGCGGCCAAAAAGGCAGGCGCTTCATTGCATAAGCCGGCCAATGCTCGCAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT79479 WP_018245323.1 NZ_CP022666:c179375-179175 [Rhizobium leguminosarum bv. viciae]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT79479 NZ_CP022666:c179375-179175 [Rhizobium leguminosarum bv. viciae]
ATGCGATCGACTATCAATCTAGACGACACTCTCATGGAAAGGGCCAGGTCCCTGACCGGCACAAAAGAAACCGCTGCTAT
AGTCCGCCAGGCGTTGGAGACGCTTGTTCGAGTAGAGTCCGGAAAACGCCTCATCGCGCTCGGCGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A444I7Z5

Antitoxin

• AlphaFold DB: A0A7Y3WNA0