Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1712818..1713455 Replicon chromosome
Accession NZ_CP022654
Organism Bacillus velezensis strain SCDB 291

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CHN56_RS08410 Protein ID WP_003156187.1
Coordinates 1712818..1713168 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CHN56_RS08415 Protein ID WP_003156188.1
Coordinates 1713174..1713455 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHN56_RS08370 1707858..1708460 - 603 WP_003156170.1 SpoIIE family protein phosphatase -
CHN56_RS08375 1708460..1709248 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CHN56_RS08380 1709214..1709696 - 483 WP_007609591.1 anti-sigma B factor RsbW -
CHN56_RS08385 1709693..1710022 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CHN56_RS08390 1710086..1711093 - 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
CHN56_RS08395 1711105..1711506 - 402 WP_003156178.1 anti-sigma regulatory factor -
CHN56_RS08400 1711509..1711874 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
CHN56_RS08405 1711879..1712700 - 822 WP_003156182.1 STAS domain-containing protein -
CHN56_RS08410 1712818..1713168 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CHN56_RS08415 1713174..1713455 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CHN56_RS08420 1713575..1714744 - 1170 WP_077721837.1 alanine racemase -
CHN56_RS08425 1714861..1715868 - 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
CHN56_RS08430 1716033..1716398 - 366 WP_070081400.1 holo-ACP synthase -
CHN56_RS08435 1716491..1717090 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T79443 WP_003156187.1 NZ_CP022654:c1713168-1712818 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T79443 NZ_CP022654:c1713168-1712818 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT79443 WP_003156188.1 NZ_CP022654:c1713455-1713174 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT79443 NZ_CP022654:c1713455-1713174 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References