Detailed information of TA system TA079442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 968503..969354
• Replicon: chromosome
• Accession: NZ_CP022654
• Organism: Bacillus velezensis strain SCDB 291

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CHN56_RS04395
• Protein ID: WP_094247019.1
• Coordinates: 968503..969183 (+)
• Length: 227 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: CHN56_RS04400
• Protein ID: WP_003154807.1
• Coordinates: 969184..969354 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHN56_RS04375	963895..964845	-	951	WP_077722102.1	ring-cleaving dioxygenase	-
CHN56_RS04380	965168..966484	+	1317	WP_094247018.1	amino acid permease	-
CHN56_RS04385	966770..967387	+	618	WP_003154804.1	DUF47 domain-containing protein	-
CHN56_RS04390	967400..968398	+	999	WP_003154805.1	inorganic phosphate transporter	-
CHN56_RS04395	968503..969183	+	681	WP_094247019.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CHN56_RS04400	969184..969354	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CHN56_RS04405	969611..970489	-	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
CHN56_RS04410	970503..970766	-	264	WP_003154813.1	phage holin	-
CHN56_RS04415	970780..971043	-	264	WP_003154815.1	hemolysin XhlA family protein	-
CHN56_RS04420	971095..971856	-	762	WP_094247020.1	phage portal protein	-
CHN56_RS04425	971913..972110	-	198	WP_071391956.1	XkdX family protein	-
CHN56_RS04430	972115..972486	-	372	WP_070081681.1	hypothetical protein	-
CHN56_RS04435	972499..974121	-	1623	WP_094247021.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-68)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 227 a.a.
• Molecular weight: 26592.46 Da
• Isoelectric Point: 4.6701

>T79442 WP_094247019.1 NZ_CP022654:968503-969183 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLAGNELETDTREI
LEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASLCDYSAQSDKDRLTEHMPDAADV
QSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLMLPIEEEGDG

Nucleotide

• Length: 681 bp

>T79442 NZ_CP022654:968503-969183 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCAGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGGCCGGCAATGAATTAGAGACGGATACGCGGGAAATT
TTAGAAGAAAATAACGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGAT
CCATGTATATTATGGAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATG
TCACGGCTTCCTTGTGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTT
CAGTCCCGTCTTAACCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCG
CCATTACGTGATCAAACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTT
ATGATTTAATGCTGCCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT79442 WP_003154807.1 NZ_CP022654:969184-969354 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT79442 NZ_CP022654:969184-969354 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14