Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 529920..530556 Replicon chromosome
Accession NZ_CP022653
Organism Bacillus atrophaeus strain GQJK17

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BaGK_RS02630 Protein ID WP_003156187.1
Coordinates 530206..530556 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag BaGK_RS02625 Protein ID WP_003328141.1
Coordinates 529920..530201 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BaGK_RS02605 526212..526811 - 600 WP_094231399.1 rhomboid family intramembrane serine protease -
BaGK_RS02610 526916..527281 + 366 WP_094231400.1 holo-ACP synthase -
BaGK_RS02615 527449..528465 + 1017 WP_094231401.1 outer membrane lipoprotein carrier protein LolA -
BaGK_RS02620 528632..529801 + 1170 WP_094231402.1 alanine racemase -
BaGK_RS02625 529920..530201 + 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BaGK_RS02630 530206..530556 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BaGK_RS02635 530672..531490 + 819 WP_003328142.1 STAS domain-containing protein -
BaGK_RS02640 531495..531860 + 366 WP_010789626.1 RsbT antagonist protein RsbS -
BaGK_RS02645 531863..532264 + 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
BaGK_RS02650 532276..533283 + 1008 WP_088116555.1 PP2C family protein-serine/threonine phosphatase -
BaGK_RS02655 533343..533672 + 330 WP_010789624.1 anti-sigma factor antagonist RsbV -
BaGK_RS02660 533669..534151 + 483 WP_003328147.1 anti-sigma B factor RsbW -
BaGK_RS02665 534117..534905 + 789 WP_003328149.1 RNA polymerase sigma factor SigB -
BaGK_RS02670 534905..535504 + 600 WP_003328150.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T79440 WP_003156187.1 NZ_CP022653:530206-530556 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T79440 NZ_CP022653:530206-530556 [Bacillus atrophaeus]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT79440 WP_003328141.1 NZ_CP022653:529920-530201 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT79440 NZ_CP022653:529920-530201 [Bacillus atrophaeus]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References