Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 176126..176769 Replicon plasmid unnamed1
Accession NZ_CP022612
Organism Klebsiella pneumoniae strain CDC 0106

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CHC10_RS27790 Protein ID WP_001044770.1
Coordinates 176353..176769 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CHC10_RS27785 Protein ID WP_001261282.1
Coordinates 176126..176356 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHC10_RS27755 171698..172564 - 867 WP_004118283.1 replication initiation protein -
CHC10_RS30470 173099..173203 + 105 WP_032409716.1 hypothetical protein -
CHC10_RS27760 173332..173589 + 258 WP_000764642.1 hypothetical protein -
CHC10_RS27765 173647..174423 - 777 WP_000015958.1 site-specific integrase -
CHC10_RS27770 174420..175163 - 744 WP_000129823.1 hypothetical protein -
CHC10_RS27775 175214..175564 - 351 WP_000493378.1 hypothetical protein -
CHC10_RS27780 175708..176169 - 462 WP_072202616.1 hypothetical protein -
CHC10_RS27785 176126..176356 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CHC10_RS27790 176353..176769 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CHC10_RS27795 176843..177553 + 711 WP_004152334.1 ATP-binding protein -
CHC10_RS27800 177587..178284 + 698 Protein_183 IS1 family transposase -
CHC10_RS27805 178369..179229 - 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
CHC10_RS27810 179412..179729 - 318 Protein_185 recombinase family protein -
CHC10_RS27815 179929..180753 - 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
CHC10_RS27820 180813..181601 - 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / aph(3')-VI / blaNDM-1 / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15 / aac(3)-IId / blaSHV-187 / ARR-3 / ere(A) / cmlA1 / aph(6)-Id / aph(3'')-Ib / sul2 - 1..334957 334957


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T79437 WP_001044770.1 NZ_CP022612:176353-176769 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T79437 NZ_CP022612:176353-176769 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT79437 WP_001261282.1 NZ_CP022612:176126-176356 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT79437 NZ_CP022612:176126-176356 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References